Akiba J
Department of Ophthalmology, Asahikawa Medical College, Japan.
Nippon Ganka Gakkai Zasshi. 1992 Jun;96(6):731-6.
Photodynamically induced vitreous liquefaction in rabbit eye was investigated. Photosensitizer, riboflavine phosphate, was injected into the vitreous cavity of the rabbit before white-light irradiation. After the irradiation (0, 1, 3, 6 hr) the rabbit vitreous body was separated into gel and liquid portions. The liquid vitreous body was weighed, and the vitreous liquefaction percentage was calculated. One hour irradiation caused 38% of liquefaction of the eye; 3 hr, 50% liquefaction; 6 hr, 59% liquefaction. Although irradiated control eye (without photosensitizer) and the dark adapted control eye (non-irradiated) showed 10-15% liquefaction throughout the experimental period, the liquefaction percentage of the experimental vitreous bodies was significantly larger than that of the control vitreous. Inhibition experiment showed that the radical scavengers (Cu, Zn-superoxide dismutase, catalase, and mannitol) could suppress the photodynamically induced vitreous liquefaction. Results indicated that free radicals, including hydroxyl radical and superoxide anion, which are generated by photosensitizer and visible light irradiation, may contribute to the age-related vitreous liquefaction of humans.
研究了光动力学诱导兔眼玻璃体液化的情况。在白光照射前，将光敏剂磷酸核黄素注入兔的玻璃体腔。照射后（0、1、3、6小时），将兔玻璃体分离为凝胶和液体部分。称取液体玻璃体的重量，并计算玻璃体液化百分比。照射1小时导致眼内38%的液化；3小时，50%液化；6小时，59%液化。尽管照射对照组眼（无光敏剂）和暗适应对照组眼（未照射）在整个实验期间显示10-15%的液化，但实验玻璃体的液化百分比明显高于对照玻璃体。抑制实验表明，自由基清除剂（铜锌超氧化物歧化酶、过氧化氢酶和甘露醇）可抑制光动力学诱导的玻璃体液化。结果表明，由光敏剂和可见光照射产生的包括羟基自由基和超氧阴离子在内的自由基可能与人类年龄相关的玻璃体液化有关。
