Matsui T, Hayashi H, Oshima K
Department of Ophthalmology, School of Medicine, Fukuoka, Japan.
Nippon Ganka Gakkai Zasshi. 1995 Feb;99(2):149-53.
To study the mechanism of vitreous liquefaction following vitreous hemorrhage, an in vitro study was done. We isolated whole vitreous gel of pig eyes, then weighed, incubated it with sample solution and shook it at room temperature. The ratio of weight loss after shaking to initial weight were calculated as vitreous liquefaction rate (VLR). There was no significant difference in VLR between samples incubated with whole blood (50.7%) and with plasma (48.8%), and it was significantly higher than control incubated with isotonic phosphate buffered physiological saline (PBS)(14.8%) after 24 hours shaking. There were no significant differences between control and samples incubated with blood cells (21.1%) and with hemoglobin (20.5%). The VLR of samples incubated with plasma gradually increased with time whereas controls incubated with PBS showed no changes until 24 hours. The VLR and concentration of plasma were highly correlated. These results indicated that contact of plasma with vitreous gel encourages liquefaction possibly by enzymatic digestive action with proteases.
为研究玻璃体积血后玻璃体液化的机制，进行了一项体外研究。我们分离出猪眼的整个玻璃体凝胶，称重后，将其与样品溶液一起孵育，并在室温下振荡。振荡后重量损失与初始重量的比值计算为玻璃体液化率（VLR）。与全血（50.7%）和血浆（48.8%）一起孵育的样品之间的VLR没有显著差异，并且在振荡24小时后，其显著高于与等渗磷酸盐缓冲生理盐水（PBS）一起孵育的对照组（14.8%）。与血细胞（21.1%）和血红蛋白（20.5%）一起孵育的样品与对照组之间没有显著差异。与血浆一起孵育的样品的VLR随时间逐渐增加，而与PBS一起孵育的对照组直到24小时都没有变化。VLR与血浆浓度高度相关。这些结果表明，血浆与玻璃体凝胶的接触可能通过蛋白酶的酶促消化作用促进液化。
