Berent Allyson C, Murakami Tsuyoshi, Scroggin Richard D, Borjesson Dori L
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN 55108, USA.
J Am Vet Med Assoc. 2005 Oct 15;227(8):1253-6. doi: 10.2460/javma.2005.227.1253.
To evaluate the clinical accuracy of reagent test strips used to estimate BUN concentration in dogs and cats.
Prospective study.
116 dogs and 58 cats.
Blood samples were collected at the time of admission to the hospital. Estimates of BUN concentration obtained with reagent test strips (category 1 [5 to 15 mg/dL], 2 (15 to 26 mg/dL], 3 [30 to 40 mg/dL], or 4 [50 to 80 mg/dL]) were compared with SUN concentrations measured with an automated analyzer. For dogs, category 1 and 2 test strip results were considered a negative result (nonazotemic) and category 3 and 4 test strip results were considered a positive result (azotemic). For cats, category 1, 2, and 3 test strip results were considered a negative result (nonazotemic) and category 4 test strip results were considered a positive result (azotemic).
On the basis of SUN concentration, 40 of the 174 (23%) animals (20 dogs and 20 cats) were classified as azotemic. One dog and 2 cats had false-negative test strip results, and 1 dog had a false-positive result. Sensitivity and specificity were 95% (20/21) and 99% (94/95), respectively, for dogs and 87% (13/15) and 100% (43/43), respectively, for cats.
Results suggest that reagent test strips are a reliable method for rapidly estimating BUN concentrations in dogs and cats. Because test strip results are only semiquantitative and there remains a potential for misclassification, especially in cats, urea nitrogen concentration should ultimately be verified by means of standard chemistry techniques.
评估用于估计犬猫血尿素氮（BUN）浓度的试剂测试条的临床准确性。
前瞻性研究。
116只犬和58只猫。
在动物入院时采集血样。将用试剂测试条获得的BUN浓度估计值（类别1[5至15mg/dL]、2[15至26mg/dL]、3[30至40mg/dL]或4[50至80mg/dL]）与用自动分析仪测量的血清尿素氮（SUN）浓度进行比较。对于犬，类别1和2测试条结果被视为阴性结果（非氮血症），类别3和4测试条结果被视为阳性结果（氮血症）。对于猫，类别1、2和3测试条结果被视为阴性结果（非氮血症），类别4测试条结果被视为阳性结果（氮血症）。
根据SUN浓度，174只动物中有40只（23%）（20只犬和20只猫）被分类为氮血症。1只犬和2只猫有测试条假阴性结果，1只犬有假阳性结果。犬的敏感性和特异性分别为95%（20/21）和99%（94/95），猫的敏感性和特异性分别为87%（13/15）和100%（43/43）。
结果表明，试剂测试条是快速估计犬猫BUN浓度的可靠方法。由于测试条结果只是半定量的，并且仍存在错误分类的可能性，尤其是在猫中，最终应通过标准化学技术验证尿素氮浓度。
