Liu Ai-lin, Zou Ya-ling, Lu Wen-hong, Wang Zeng-zhen, Lu Wen-qing
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005 Oct;23(5):343-6.
To investigate the application of serum glutathione S-transferase (GST) and urinary 8-hydroxy-2-deoxyguanosine (8-OHdG) as the monitoring biomarkers for coke oven workers exposed to polycyclic aromatic hydrocarbons (PAHs).
47 male coke oven workers and 31 male control workers were investigated. Urinary 8-OHdG and serum GST were analyzed using high performance liquid chromatography (HPLC) with electrochemical detection and test kit. Urinary 1-hydroxypyrene (1-OHP) as internal exposure of PAHs was also determined simultaneously by alkaline hydrolysis and HPLC.
The values of urinary 1-OHP, serum GST and urinary 8-OHdG were reported as median with interquartile range (P(25)-P(75)). Urinary 1-OHP [5.7 (1.4-12.0) micromol/mol Cr], serum GST [22.1 (14.9-31.2) U/ml], and urinary 8-OHdG [1.9 (1.4-15.4) micromol/mol Cr] in coke oven workers were significantly higher than in control workers [3.0 (0.5-6.4) micromol/mol Cr (P < 0.05), 13.1 (9.5-16.7) U/ml (P < 0.01), and 1.3 (1.0-4.0) micromol/mol Cr (P < 0.05) respectively]. Categorizing by smoking status, significant differences in urinary 1-OHP and serum GST were found only in smokers among coke oven workers compared to control workers (P < 0.01), and 8-OHdG levels only in non-smokers (P < 0.01). Additionally, there was significant correlation between urinary 1-OHP and serum GST activity (r(s) = 0.31, P < 0.01, n = 78). The multiple logistic regression analysis showed that coke oven workers were at the higher risk of having GST activities above 16.7 U/ml (OR = 13.2) and 8-OHdG levels above 1.8 micromol/mol creatinine (OR = 4.4). High body mass index was an independent factor to affect urinary 8-OHdG levels.
The elevated serum GST activities and increased oxidative DNA damage were found in the coke oven workers. Occupational exposure and smoking interact on each other. Serum GST may be used as a biomarker for assessing the exposure of PAHs. Assay of urinary 8-OHdG may be useful for evaluating the risk of lung cancer in coke oven workers.
探讨血清谷胱甘肽S - 转移酶（GST）和尿8 - 羟基 - 2 - 脱氧鸟苷（8 - OHdG）作为多环芳烃（PAHs）暴露焦炉工人监测生物标志物的应用。
调查47名男性焦炉工人和31名男性对照工人。采用高效液相色谱（HPLC）电化学检测法和试剂盒分析尿8 - OHdG和血清GST。同时采用碱性水解和HPLC法测定尿中1 - 羟基芘（1 - OHP）作为PAHs的内暴露指标。
尿1 - OHP、血清GST和尿8 - OHdG的值以中位数和四分位数间距（P(25) - P(75)）报告。焦炉工人尿1 - OHP[5.7（1.4 - 12.0）μmol/mol Cr]、血清GST[22.1（14.9 - 31.2）U/ml]和尿8 - OHdG[1.9（1.4 - 15.4）μmol/mol Cr]显著高于对照工人[分别为3.0（0.5 - 6.4）μmol/mol Cr（P < 0.05）、13.1（9.5 - 16.7）U/ml（P < 0.01）和1.3（1.0 - 4.0）μmol/mol Cr（P < 0.05）]。按吸烟状况分类，与对照工人相比，仅在焦炉工人中的吸烟者尿1 - OHP和血清GST有显著差异（P < 0.01），而8 - OHdG水平仅在非吸烟者中有显著差异（P < 0.0）。此外，尿1 - OHP与血清GST活性之间存在显著相关性（r(s) = 0.31，P < 0.01，n = 78）。多因素logistic回归分析显示，焦炉工人血清GST活性高于16.7 U/ml（OR = 13.2）和8 - OHdG水平高于1.8 μmol/mol肌酐（OR = 4.4）的风险更高。高体重指数是影响尿8 - OHdG水平的独立因素。
焦炉工人血清GST活性升高且氧化DNA损伤增加。职业暴露与吸烟相互作用。血清GST可作为评估PAHs暴露的生物标志物。检测尿8 - OHdG可能有助于评估焦炉工人患肺癌的风险。
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