de Boer N, Kroese F G, Sharp J G, Perry G A
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Anat Rec. 1992 Aug;233(4):569-76. doi: 10.1002/ar.1092330411.
Proximal colonic lymphoid tissue (PCLT) is a lymphoid structure located in the proximal colon of the mouse and the rat. In the present investigation we studied the immunomorphology and cytology of PCLT in the rat. We also studied sites of lymphocyte proliferation using the BrdU-anti BrdU technique. Results demonstrated no evident phenotypical differences between the lymphocyte populations of PCLT and either jejunal or ileal Peyer's patches (PP). The majority of the lymphocytes within PCLT were B cells localized in follicles, which were separated from each other by interfollicular T cell areas. Germinal centers (GC), containing ED5+ follicular dendritic cells, are found within PCLT follicles. The T cell areas contained both MHC Class II+ interdigitating cells and high endothelial venules. Studies using BrdU-anti BrdU indicated that lymphocyte proliferation within PCLT takes place mainly in germinal centers. Together the data show that the organization, lymphoid constituents, and sites of lymphocyte production are very similar in PCLT and PP. We therefore conclude that PCLT in the rat is not a Bursa equivalent, but more likely a PP with some special characteristics.
近端结肠淋巴组织（PCLT）是位于小鼠和大鼠近端结肠的一种淋巴结构。在本研究中，我们研究了大鼠PCLT的免疫形态学和细胞学。我们还使用BrdU-抗BrdU技术研究了淋巴细胞增殖的部位。结果表明，PCLT的淋巴细胞群体与空肠或回肠派伊尔结（PP）的淋巴细胞群体之间没有明显的表型差异。PCLT内的大多数淋巴细胞是位于滤泡中的B细胞，滤泡之间被滤泡间T细胞区域分隔开。在PCLT滤泡内发现含有ED5 +滤泡树突状细胞的生发中心（GC）。T细胞区域含有MHC II类+交错突细胞和高内皮微静脉。使用BrdU-抗BrdU的研究表明，PCLT内的淋巴细胞增殖主要发生在生发中心。这些数据共同表明，PCLT和PP在组织结构、淋巴成分和淋巴细胞产生部位方面非常相似。因此，我们得出结论，大鼠的PCLT不是法氏囊的等效物，而更可能是具有一些特殊特征的PP。
