Soesatyo M, van den Berg T K, van Rees E P, Biewenga J, Sminia T
Department of Cell Biology, Faculty of Medicine, Vrije Universiteit, Amsterdam, The Netherlands.
Reg Immunol. 1992 Jan-Feb;4(1):46-52.
Ontogeny of reticulum cells (RC) in the rat intestine in relation to the development of the gut-associated lymphoid tissue (GALT) was studied using a panel of monoclonal antibodies (mab) directed against RC in peripheral lymphoid organs, ED10-ED15. The mab ED10 specific for RC in the spleen T cell area, recognized an epitope on gut RC, which cells seem to be involved in the influx and accumulation of lymphocytes in the lamina propria and in Peyer's patches (PP) and proximal colonic lymphoid tissue (PCLT). The mab ED11 which recognizes RC in the T cell area and B cell follicles of spleen, stained follicular dendritic cells (FEC) in the B cell area of the mesenteric lymph node (MLN), PP and PCLT. The ED11 expression occurs earlier and reveals stronger staining in MLN as compared to those in PP and PCLT, suggesting the prominent role of MLN in the generation and proliferation of B cells in the gut mucosal immune system. The mab ED13 specific for RC in the B cell area of the lymph nodes, stained the basement membrane of the epithelium overlying PP and PCLT, and high endothelial venules (HEV), suggesting that this might be involved in providing the microenvironment for the development and differentiation of follicle-associated epithelium, and facilitating lymphocyte traffic. The mab ED12 specific for RC in the paracortex of peripheral lymph nodes, gave a diffuse nonspecific staining in the gut, whereas mab ED14 and ED15 are markers for common connective tissue cells. We conclude that the gut RC are morphologically and phenotypically heterogenous. ED10+, ED11+, and ED13+ RC are probably involved in the development of the gut lymphoid microenvironment.
利用一组针对外周淋巴器官（ED10 - ED15）中网状细胞（RC）的单克隆抗体，研究了大鼠肠道中网状细胞的个体发生与肠道相关淋巴组织（GALT）发育的关系。特异性针对脾脏T细胞区中RC的单克隆抗体ED10，识别肠道RC上的一个表位，这些细胞似乎参与淋巴细胞在固有层、派伊尔结（PP）和近端结肠淋巴组织（PCLT）中的流入和积聚。识别脾脏T细胞区和B细胞滤泡中RC的单克隆抗体ED11，对肠系膜淋巴结（MLN）、PP和PCLT的B细胞区中的滤泡树突状细胞（FDC）进行染色。与PP和PCLT相比，ED11在MLN中的表达出现得更早且染色更强，表明MLN在肠道黏膜免疫系统中B细胞的生成和增殖中起重要作用。特异性针对淋巴结B细胞区中RC的单克隆抗体ED13，对覆盖PP和PCLT的上皮基底膜以及高内皮微静脉（HEV）进行染色，表明这可能参与为滤泡相关上皮的发育和分化提供微环境，并促进淋巴细胞运输。特异性针对外周淋巴结副皮质区中RC的单克隆抗体ED12，在肠道中呈弥漫性非特异性染色，而单克隆抗体ED14和ED15是常见结缔组织细胞的标志物。我们得出结论，肠道RC在形态和表型上是异质的。ED10 +、ED11 +和ED13 +的RC可能参与肠道淋巴微环境的发育。
