Ohgami M, Doerschuk C M, Gie R P, English D, Hogg J C
University of British Columbia Pulmonary Research Laboratory, Vancouver, Canada.
Am Rev Respir Dis. 1992 Jul;146(1):190-5. doi: 10.1164/ajrccm/146.1.190.
Recent studies from our laboratory show that the lung contains a marginated pool of monocytes. The present study was designed to investigate monocyte accumulation in this pool 4 to 28 h after a single dose of endotoxin when the endotoxin had disappeared from the circulation. This was accomplished by administering a single intravenous dose of endotoxin (Escherichia coli, 50 micrograms/rabbit) to unanesthetized animals (n = 6) and saline to controls (n = 5) at time 0. Four hours after this dose of endotoxin, 111In-monocytes (93.5% pure) isolated from donors were injected intravenously, and, at 27 h, the rabbits were anesthetized and colloidal carbon (CC, 1 ml/kg body weight) was injected intraarterially to provide a phagocytic stimulus. The animals were sacrificed at 28 h, and the lungs were fixed in situ with glutaraldehyde. The data show that lungs from the endotoxin-treated rabbits contained 4.8 times more 111In-monocytes than controls, that 92% of these radiolabeled monocytes were in the alveolar capillaries, and that 72% of these labeled cells had phagocytosed CC. The histologic studies of unlabeled cells confirmed that this endotoxin treatment caused a 3-5-fold increase in unlabeled mononuclear cells and neutrophils (PMN) in the microvasculature and that many of the unlabeled monocytes in the endotoxin-treated group had also phagocytosed colloidal carbon. The behavior of the donor monocytes injected after the endotoxin had time to disappear from the circulation suggests that they accumulate in the lung in response to the indirect effects of endotoxin on endothelial cells.
我们实验室最近的研究表明，肺中存在一个单核细胞边缘池。本研究旨在调查单次注射内毒素后4至28小时，当内毒素已从循环中消失时，单核细胞在该池中积聚的情况。这是通过在时间0给未麻醉的动物（n = 6）静脉注射单次剂量的内毒素（大肠杆菌，50微克/兔），给对照组（n = 5）注射生理盐水来完成的。在该剂量的内毒素注射后4小时，静脉注射从供体分离的111In标记单核细胞（纯度93.5%），并在27小时时，将兔子麻醉并动脉内注射胶体碳（CC，1毫升/千克体重）以提供吞噬刺激。在28小时处死动物，并用戊二醛原位固定肺。数据显示，内毒素处理组兔子的肺中111In标记单核细胞比对照组多4.8倍，这些放射性标记单核细胞的92%位于肺泡毛细血管中，并且这些标记细胞的72%已吞噬CC。对未标记细胞的组织学研究证实，这种内毒素处理导致微血管中未标记的单核细胞和中性粒细胞（PMN）增加3至5倍，并且内毒素处理组中的许多未标记单核细胞也吞噬了胶体碳。在内毒素从循环中消失后注射的供体单核细胞的行为表明，它们因内毒素对内皮细胞的间接作用而在肺中积聚。
