Doerschuk C M, Allard M F, Martin B A, MacKenzie A, Autor A P, Hogg J C
University of British Columbia, Pulmonary Research Laboratory, Vancouver, Canada.
J Appl Physiol (1985). 1987 Nov;63(5):1806-15. doi: 10.1152/jappl.1987.63.5.1806.
The size and location of the marginated pool of neutrophils (PMNs) in rabbit lungs were evaluated, and the rate of exchange of the PMNs with the circulating pool was determined. 99mTc-labeled erythrocytes (99mTc-RBCs) and 125I-labeled macroaggregated albumin (125I-MAA) were used to determine RBC transit times in the pulmonary circulation. Radiolabeled PMNs were studied on their first passage through the lungs. After 10 min of circulation, the lungs were fixed, gamma counted, and prepared for morphometric and autoradiographic studies; 74 +/- 3% of the PMNs was retained in the lungs on the first passage, and 23 +/- 2% was within the pulmonary marginated pool 10 min later. The regional PMN retention and the rate of exchange between the marginated and circulating PMN pools in the lung were directly related to RBC transit time. The radiolabeled PMNs distributed similarly to the unlabeled cells within the microvasculature and had a similar exchange rate between the marginated and circulating pools (1.4 +/- 0.2%/s using labeled cells and 1.5 +/- 0.5%/s using unlabeled cells). The marginated pool was located primarily within alveolar capillaries and contained two to three times as many PMNs as the total circulating pool.
对兔肺中嗜中性粒细胞（PMN）边缘池的大小和位置进行了评估，并测定了PMN与循环池之间的交换率。使用99mTc标记的红细胞（99mTc-RBC）和125I标记的大颗粒白蛋白（125I-MAA）来测定肺循环中红细胞的通过时间。对放射性标记的PMN首次通过肺的情况进行了研究。循环10分钟后，将肺固定、进行γ计数，并准备进行形态计量学和放射自显影研究；首次通过时74±3%的PMN滞留在肺中，10分钟后23±2%的PMN位于肺边缘池内。肺中局部PMN的滞留以及边缘池与循环PMN池之间的交换率与红细胞通过时间直接相关。放射性标记的PMN在微血管内的分布与未标记细胞相似，在边缘池与循环池之间的交换率也相似（使用标记细胞时为1.4±0.2%/秒，使用未标记细胞时为1.5±0.5%/秒）。边缘池主要位于肺泡毛细血管内，所含PMN数量是总循环池的两到三倍。
