Seiffert S, Oppermann W
Institute of Physical Chemistry, Clausthal University of Technology, Clausthal-Zellerfeld, Germany.
J Microsc. 2005 Oct;220(Pt 1):20-30. doi: 10.1111/j.1365-2818.2005.01512.x.
The diffusion coefficient as well as the dimensionality of the diffusion process can be determined by straightforward and facile data analysis, when fluorescence recovery after photobleaching (FRAP) is measured as a function of time and space by means of confocal laser scanning microscopy. Experiments representing one-dimensional diffusion from a plane source or two-dimensional diffusion from a line source are readily realized. In the data analysis, the deviations of the actual initial conditions from ideal models are consistently taken into account, so that no calibration measurements are needed. The method is applied to FRAP experiments on solutions of Rhodamine B in glycerol and aqueous suspensions of polymethyl methacrylate microspheres.
当通过共聚焦激光扫描显微镜测量光漂白后荧光恢复（FRAP）作为时间和空间的函数时，扩散系数以及扩散过程的维度可以通过直接且简便的数据分析来确定。代表从平面源进行一维扩散或从线源进行二维扩散的实验很容易实现。在数据分析中，始终会考虑实际初始条件与理想模型的偏差，因此无需进行校准测量。该方法应用于罗丹明B在甘油溶液和聚甲基丙烯酸甲酯微球水悬浮液的FRAP实验。
