Ito Yoshinori, Okada Yuji, Sato Mikinori, Sawai Hirozumi, Funahashi Hitoshi, Murase Takayuki, Hayakawa Tetsushi, Manabe Tadao
Department of Gastroenterological Surgery, Nagoya City University, Graduate School of Medical Sciences, Kawasumi, Japan.
Surgery. 2005 Oct;138(4):788-94. doi: 10.1016/j.surg.2005.07.007.
The glial cell line-derived neurotrophic factor (GDNF) is a member of neurotrophic polypeptide family, which promotes survival and rescue of various neural cells in the central and peripheral nerve systems. We previously reported that GDNF promotes tumor cell invasion in pancreatic cancer cell lines. The purpose of this study was to investigate GDNF family expression and the status of related receptors in actual cancer tissues, and assess correlations with clinicopathologic behavior.
Immunohistochemical assessment of GDNF, neurturin, persephin, artemin, GDNF family receptor alpha-1 and alpha-2, and RET was performed for 51 cases of surgically resected pancreatic cancer.
In all intrapancreatic nerves, GDNF and artermin were expressed strongly. In pancreatic cancer tissues. The expression of RET was stronger than that seen in normal ductal cells and was significantly related to the survival rate after resection (P = .026) and lymphatic invasion (P = .014). Intrapancreatic neural invasion was significantly related to the expression of GDNF (P = .047).
We conclude that the expression of RET in pancreatic cancer tissues may be a useful prognostic marker and GDNF may play an important role in neural invasion.
胶质细胞源性神经营养因子(GDNF)是神经营养多肽家族的成员,可促进中枢和周围神经系统中各种神经细胞的存活和挽救。我们之前报道过GDNF可促进胰腺癌细胞系中的肿瘤细胞侵袭。本研究的目的是调查GDNF家族在实际癌组织中的表达及相关受体的状态,并评估其与临床病理行为的相关性。
对51例手术切除的胰腺癌进行GDNF、神经营养因子、帕西芬、Artemin、GDNF家族受体α-1和α-2以及RET的免疫组织化学评估。
在所有胰腺内神经中,GDNF和Artemin表达强烈。在胰腺癌组织中,RET的表达强于正常导管细胞,且与切除后的生存率(P = 0.026)和淋巴侵袭(P = 0.014)显著相关。胰腺内神经侵袭与GDNF的表达显著相关(P = 0.047)。
我们得出结论,RET在胰腺癌组织中的表达可能是一个有用的预后标志物,且GDNF可能在神经侵袭中起重要作用。