Pusterla Nicola, Holmberg Tara A, Lorenzo-Figueras Mireia, Wong Anita, Wilson W David
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California-Davis, CA, USA.
Vet Clin Pathol. 2005 Dec;34(4):413-6. doi: 10.1111/j.1939-165x.2005.tb00072.x.
A 10-year-old Thoroughbred gelding was admitted to the Veterinary Medical Teaching Hospital of the University of California-Davis with a 2-week history of intermittent fever and acute onset of lethargy, anorexia, and ataxia. Although the clinical signs were nonspecific, the results of initial hematologic and biochemical analysis were consistent with a chronic inflammatory process. Thoracic radiographs revealed an increased fine reticulonodular interstitial opacity throughout the dorsal caudal lung fields. Cytologic examination of bronchoalveolar lavage (BAL) fluid showed mixed inflammation with many mononuclear phagocytes containing single, spherical, intracytoplasmic fungal organisms. Four mold species were cultured in low numbers from the BAL fluid. One of the fungal elements observed on the culture plates was identified as Acremonium strictum by real-time polymerase chain reaction (PCR). A diagnosis of fungal pneumonia due to A strictum was made based on the results of thoracic imaging, cytologic evaluation, culture, and PCR testing. The horse made an uneventful recovery with supportive treatment and was disease-free based on normal physical, radiographic, and cytologic findings at 21 days after presentation. To our knowledge, this is the first report of isolation of A strictum from the BAL fluid of a horse with interstitial pneumonia.
一匹10岁的纯种马被收治于加利福尼亚大学戴维斯分校兽医学院教学医院，有两周间歇性发热病史，并急性起病出现嗜睡、厌食和共济失调。尽管临床症状无特异性，但初始血液学和生化分析结果与慢性炎症过程一致。胸部X光片显示整个背侧尾叶肺野细网状结节状间质混浊增加。支气管肺泡灌洗（BAL）液的细胞学检查显示混合性炎症，有许多单核吞噬细胞含有单个球形胞浆内真菌生物体。从BAL液中培养出四种霉菌，数量较少。通过实时聚合酶链反应（PCR）在培养平板上观察到的一种真菌成分被鉴定为曲霉。根据胸部影像学、细胞学评估、培养和PCR检测结果，诊断为曲霉引起的真菌性肺炎。这匹马经支持治疗后顺利康复，在就诊21天后，根据正常的体格检查、影像学和细胞学检查结果未发现疾病。据我们所知，这是首次从一匹间质性肺炎马的BAL液中分离出曲霉的报告。
