Hung Chen-Road
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan, China.
World J Gastroenterol. 2005 Oct 7;11(37):5853-8. doi: 10.3748/wjg.v11.i37.5853.
To evaluate the protective effect of lysozyme chloride on betel quid chewing (BQC) aggravated gastric oxidative stress and hemorrhagic ulcer in rats with diabetes mellitus (DM).
Male Wistar rats were challenged intravenously with streptozotocin (65 mg/kg) to induce DM. Rats were fed with regular pellet food or BQC-containing diets. After 90 d, rats were deprived of food for 24 h. Rat stomachs were irrigated for 3 h with normal saline or simulated gastric juice. Rats were killed and gastric specimens were harvested.
An enhancement of various gastric ulcerogenic parameters, including acid back-diffusion, mucosal lipid peroxide generation, as well as decreased glutathione levels and mucus content, were observed in DM rats. After feeding DM rats with BQC, an exacerbation of these ulcero-genic parameters was achieved. Gastric juice caused a further aggravation of these ulcerogenic parameters. Daily intragastric lysozyme chloride dose-dependently inhibited exacerbation of various ulcerogenic parameters in those BQC-fed DM rats.
(1) Gastric juice could aggravate both DM and BQC-fed DM rat hemorrhagic ulcer; (2) BQC exacerbated gastric hemorrhagic ulcer in DM rats via enhancing oxidative stress and reducing defensive factors; (3) lysozyme chloride effectively protected BQC aggravated gastric damage in DM rats.
评估氯化溶菌酶对槟榔咀嚼（BQC）加重糖尿病（DM）大鼠胃氧化应激和出血性溃疡的保护作用。
雄性Wistar大鼠静脉注射链脲佐菌素（65 mg/kg）诱导DM。大鼠喂食常规颗粒饲料或含BQC的饲料。90天后，大鼠禁食24小时。用生理盐水或模拟胃液冲洗大鼠胃3小时。处死大鼠并采集胃标本。
在DM大鼠中观察到各种胃溃疡形成参数增强，包括酸反流、黏膜脂质过氧化物生成增加，以及谷胱甘肽水平和黏液含量降低。给DM大鼠喂食BQC后，这些溃疡形成参数进一步恶化。胃液导致这些溃疡形成参数进一步加重。每日胃内给予氯化溶菌酶剂量依赖性地抑制了那些喂食BQC的DM大鼠中各种溃疡形成参数的恶化。
（1）胃液可加重DM和喂食BQC的DM大鼠的出血性溃疡；（2）BQC通过增强氧化应激和减少防御因子加重DM大鼠的胃出血性溃疡；（3）氯化溶菌酶有效保护喂食BQC的DM大鼠的胃损伤。
