Wang Jong-Shyan, Chen Yu-Wen, Chow Shu-Er, Ou Hsiu-Chung, Sheu Wayne Huey-Herng
Graduate Institute of Rehabilitation Science and Center for Gerontological Research, Chang Gung University, Tao-Yuan, Taiwan.
Thromb Haemost. 2005 Oct;94(4):846-52. doi: 10.1160/TH05-02-0139.
Physical exercise can affect the risk of cardiovascular disease. Oxidized-low density lipoprotein (ox-LDL) promotes transendothelial migration (TEM) of monocyte, thereby accelerating the pathogenesis of atherosclerosis. This study investigated how exercise intensity affects monocyte/EC interactions under ox-LDL-mediated condition. Light- (LIE), moderate- (MIE) and high- (HIE) intensity exercise (i.e., 40%, 60%, and 80% VO2max, respectively) on a bicycle ergometer in 18 sedentary healthy men were performed on three separate occasions. Before and immediately after exercise, ox-LDL-promoted expressions of monocyte adhesion molecules and TEM of monocyte, as well as oxidation of LDL and amounts of soluble adhesion molecules in plasma were measured. Analytical results showed that (1) ox-LDL furthered monocyte L-selectin shedding and Mac-1 expression, and an attendant increase in TEM of monocyte, while treating the monocyte with Mac-1 antibody inhibited the ox-LDL-promoted TEM of monocyte; (2) under ox-LDL-treated condition, MIE increased monocyte Mac-1 and LFA-1 expressions, enhancing the TEM of monocyte, whereas HIE downregulated monocyte Mac-1 expression, suppressing the TEM of monocyte; (3) LIE decreased basal LFA-1 expression as well as basal and ox-LDL-promoted TEM of monocyte; and (4) MIE and HIE, but not LIE, elevated plasma ox-LDL level, while there were no significant changes in sL-selectin, sE-selectin, sICAM-1, and sVCAM-1 following these exercises. Therefore, we conclude that monocyte activation and subsequent TEM promoted by ox-LDL are changed by short-term exercise in an intensity-dependent manner. These findings provide a new insight into the may aid the development of suitable exercise intensity enable people to prevent early atherogenesis.
体育锻炼会影响心血管疾病的风险。氧化型低密度脂蛋白（ox-LDL）可促进单核细胞的跨内皮迁移（TEM），从而加速动脉粥样硬化的发病机制。本研究调查了运动强度如何在ox-LDL介导的条件下影响单核细胞/内皮细胞的相互作用。对18名久坐不动的健康男性在自行车测力计上分别进行了三次不同强度的运动，即轻度（LIE）、中度（MIE）和高强度（HIE）运动（分别为最大摄氧量的40%、60%和80%）。在运动前和运动后立即测量ox-LDL促进的单核细胞黏附分子表达、单核细胞的TEM，以及血浆中LDL的氧化和可溶性黏附分子的含量。分析结果表明：（1）ox-LDL促进单核细胞L-选择素脱落和Mac-1表达，并伴随单核细胞TEM增加，而用Mac-1抗体处理单核细胞可抑制ox-LDL促进的单核细胞TEM；（2）在ox-LDL处理条件下，MIE增加单核细胞Mac-1和LFA-1表达，增强单核细胞的TEM，而HIE下调单核细胞Mac-1表达，抑制单核细胞的TEM；（3）LIE降低基础LFA-1表达以及基础和ox-LDL促进的单核细胞TEM；（4）MIE和HIE，但不是LIE，提高血浆ox-LDL水平，而这些运动后sL-选择素、sE-选择素、sICAM-1和sVCAM-1无显著变化。因此，我们得出结论，ox-LDL促进的单核细胞激活和随后的TEM会因短期运动而以强度依赖的方式发生改变。这些发现为预防早期动脉粥样硬化的适宜运动强度的制定提供了新的见解。
