Guereschi Marcia G, Prestes Jonato, Donatto Felipe F, Dias Rodrigo, Frollini Anelena B, Ferreira Clílton Ko, Cavaglieri Claudia R, Palanch Adrianne C
Health Sciences Faculty, Methodist University of Piracicaba, São Paulo, BRAZIL.
Health Sciences Faculty, Methodist University of Piracicaba, São Paulo, BRAZIL; Physiological Sciences Department, Exercise Physiology Laboratory, Federal University of São Carlos, São Paulo, BRAZIL.
Int J Exerc Sci. 2008 Apr 15;1(2):71-78. doi: 10.70252/JNVX6872. eCollection 2008.
The purpose of this study was to verify the histophysiological alterations in monocytes and macrophages induced by short periods of exercise. Male Wistar rats (age = 2 months, body weight = 200g) were divided into seven groups (N = 6 each): sedentary control (C), groups exercised (swimming) at low intensity for 5 (5L), 10 (10L), and 15 minutes (15L), and groups exercised at moderate intensity for 5 (5M), 10 (10M) or 15 minutes (15M). At moderate intensity the animals carried a load of 5% of body weight on their backs. Blood monocytes were evaluated for quantity and morphology, and peritoneal macrophages were analyzed for quantity and phagocytic activity. Data were analyzed using ANOVA and Tukey's post hoc test (p ≤ 0.05). Low intensity groups and 5M exhibited an increase in monocyte levels when compared with the control. There was an increase in monocyte cellular area for the 5L, 10L, 5M and 10M groups; monocyte nuclear area increased for the 10L, 5M and 10M groups in comparison with the control. There was an increase in peritoneal macrophages for the 15L, 10M, 15M and decrease for the 5M group. Macrophage phagocytic capacity increased for low intensity groups and for 10M group. The exercise performed for short periods modulated macrophage levels and function, and monocyte levels and morphology, in an intensity-dependent manner. The sum of acute responses observed in this study may exert a protective effect against sickness and may be used to improve health and lifespan.
本研究的目的是验证短时间运动诱导的单核细胞和巨噬细胞的组织生理变化。雄性Wistar大鼠（年龄 = 2个月，体重 = 200g）被分为七组（每组N = 6）：久坐对照组（C）、低强度运动（游泳）5分钟（5L）、10分钟（10L）和15分钟（15L）的组，以及中等强度运动5分钟（5M）、10分钟（10M）或15分钟（15M）的组。在中等强度运动时，动物背部负重为体重的5%。评估血液单核细胞的数量和形态，并分析腹腔巨噬细胞的数量和吞噬活性。数据采用方差分析和Tukey事后检验进行分析（p≤0.05）。与对照组相比，低强度组和5M组的单核细胞水平有所增加。5L、10L、5M和10M组的单核细胞细胞面积增加；与对照组相比，10L、5M和10M组的单核细胞核面积增加。15L、10M、15M组的腹腔巨噬细胞增加，而5M组减少。低强度组和10M组的巨噬细胞吞噬能力增加。短时间进行的运动以强度依赖的方式调节巨噬细胞水平和功能，以及单核细胞水平和形态。本研究中观察到的急性反应总和可能对疾病起到保护作用，并可用于改善健康和延长寿命。
