Witczak Carol A, Mokelke Eric A, Boullion Robert, Wenzel James, Keisler Duane H, Sturek Michael
Department of Medical Pharmacology & Physiology, School of Medicine, University of Missouri, Columbia, Missouri, USA.
Comp Med. 2005 Oct;55(5):445-51.
The purpose of this study was to assess the use of body circumference, ultrasonography, and serum leptin levels as noninvasive measures to estimate body fat percentage in adult, male, Yucatan swine, which are widely used in biomedical research models. Swine (ages 8 to 15 months) were maintained for 20 weeks: control (n = 7); high-fat, high-cholesterol diet (hyperlipidemic; n = 8); alloxan-induced diabetes with high-fat, high-cholesterol diet (diabetic dyslipidemic; n = 7); and diabetic dyslipidemic plus exercise-trained (n = 6). Anesthetized swine were positioned on their dorsum for the following measurements: 1) neck, mid-abdomen, and widest abdominal girth circumferences; and 2) neck and mid-abdomen ultrasound measurements. Blood samples were obtained for quantification of serum leptin levels. After euthanasia, the carcass and viscera were separated for chemical composition analysis, which demonstrated a significant increase in carcass and visceral fat in the diabetic dyslipidemic swine compared to controls. Serum leptin levels were also increased in the hyperlipidemic and diabetic dyslipidemic swine. Regression analyses demonstrated a significant correlation between carcass fat, visceral fat, and all of the circumference, ultrasound, and serum leptin measures. In conclusion, the widest abdominal girth circumference was the noninvasive measure with the highest predictive value for estimating carcass and visceral fat in adult, male Yucatan miniature swine.
本研究的目的是评估使用体围、超声检查和血清瘦素水平作为非侵入性测量方法，来估计成年雄性尤卡坦猪的体脂百分比，这种猪在生物医学研究模型中被广泛使用。猪（8至15个月龄）饲养20周：对照组（n = 7）；高脂高胆固醇饮食组（高脂血症；n = 8）；高脂高胆固醇饮食诱导的四氧嘧啶糖尿病组（糖尿病血脂异常；n = 7）；糖尿病血脂异常加运动训练组（n = 6）。将麻醉后的猪仰卧位进行以下测量：1）颈部、中腹部和最宽腹围；2）颈部和中腹部超声测量。采集血样以定量血清瘦素水平。安乐死后，将 carcass 和内脏分离进行化学成分分析，结果表明糖尿病血脂异常猪的 carcass 和内脏脂肪与对照组相比显著增加。高脂血症和糖尿病血脂异常猪的血清瘦素水平也升高。回归分析表明 carcass 脂肪、内脏脂肪与所有体围、超声和血清瘦素测量值之间存在显著相关性。总之，在成年雄性尤卡坦小型猪中，最宽腹围是估计 carcass 和内脏脂肪预测价值最高的非侵入性测量方法。 （注：carcass 直译为“胴体”，这里根据语境灵活翻译为合适的表述）
