Kuimov A N, Konareva N V, Kochetov G A
A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia.
Biochem Int. 1992 Mar;26(3):451-5.
Monocrystals of three individual multiple forms of yeast transketolase (A, B and C) differing in their thermostability have been obtained. Ammonium sulfate was used as a precipitating agent. Crystals of the mentioned forms were found to possess different morphology and stability during storage. Single crystals growing from the enzyme form C within 4-7 days were subsequently destroyed. Simultaneously, in the preparation, microcrystals started to grow in a great number. They were found to correspond morphologically to crystals obtained from transketolase A. A possibility of interconversions of the enzyme forms in sequence C----A----B is discussed.
已获得三种热稳定性不同的酵母转酮醇酶（A、B和C）单一多重形式的单晶。硫酸铵用作沉淀剂。发现上述形式的晶体在储存期间具有不同的形态和稳定性。从酶形式C在4 - 7天内生长出的单晶随后被破坏。同时，在制剂中大量微晶开始生长。发现它们在形态上与从转酮醇酶A获得的晶体相对应。讨论了酶形式按C→A→B顺序相互转化的可能性。
