含有延长的夏因-达尔加诺序列的信使核糖核酸（mRNA）的翻译独立于核糖体蛋白S1。

mRNA containing an extended Shine-Dalgarno sequence is translated independently of ribosomal protein S1.

作者信息

Balakin A G, Bogdanova S L, Skripkin E A

机构信息

Moscow State University, Russia.

出版信息

Biochem Int. 1992 Jun;27(1):117-29.

PMID:1627169

Abstract

The role of ribosomal protein S1 in the translation of mRNA containing an extended Shine-Dalgarno sequence was investigated. Using the toeprinting technique, formation of the ternary initiation complex between 30S subunits, both S1-depleted or treated with anti-S1 antibodies, and mini-mRNA containing the 9 nucleotide-long Shine-Dalgarno sequence was studied. It was concluded that the initiation of translation on mRNA with an extended Shine-Dalgarno sequence is S1-independent. It was demonstrated that S1-depleted ribosomes effectively translate the cro-mini-mRNA in a cell-free system. In contrast to cro-mini-mRNA, 30S subunits without protein S1 are inactive in ternary initiation complex formation with, and cell-free translation of, MS2 or fr phage RNAs and RNA protein III of phage fd.

摘要

研究了核糖体蛋白S1在含有延长的Shine-Dalgarno序列的mRNA翻译中的作用。使用足迹法，研究了30S亚基（无论是S1缺失的还是用抗S1抗体处理的）与含有9个核苷酸长的Shine-Dalgarno序列的微型mRNA之间三元起始复合物的形成。得出的结论是，具有延长的Shine-Dalgarno序列的mRNA上的翻译起始不依赖于S1。结果表明，缺失S1的核糖体在无细胞系统中能有效地翻译cro-微型mRNA。与cro-微型mRNA不同，没有蛋白S1的30S亚基在与MS2或fr噬菌体RNA以及噬菌体fd的RNA蛋白III形成三元起始复合物和无细胞翻译中是无活性的。