Balakin A G, Bogdanova S L, Skripkin E A
Biokhimiia. 1993 Jan;58(1):98-107.
The role of the ribosomal protein S1 in translation of a mRNA containing an extended Shine-Dalgarno sequence has been investigated. Using the toe printing technique, the formation of a ternary initiation complex with both S1-depleted 30S subunits and subunits treated with anti-S1 antibodies and mini-mRNA containing an lambda-cro-mRNA translational initiation region with a very long (9 nucleotides) Shine-Dalgarno sequence, has been demonstrated. It is concluded that initiation of translation on mRNA with extended SD-sequence is S1-independent. By means of an E. coli cell-free system of translation (S-100 extract), the translation of mini-cro-mRNA by S1-depleted ribosomes has been shown. In contrast with mini-cro-mRNA, the 30S subunits without protein S1 are inactive both in the ternary initiation complex formation and in cell-free translation with MS2 or fr phage RNAs and RNA protein III of phage fd.
核糖体蛋白S1在含有延伸的Shine-Dalgarno序列的mRNA翻译中的作用已被研究。使用足迹技术，已证明与不含S1的30S亚基以及用抗S1抗体处理的亚基和含有具有非常长（9个核苷酸）Shine-Dalgarno序列的λ-cro-mRNA翻译起始区域的微型mRNA形成三元起始复合物。得出的结论是，具有延伸SD序列的mRNA上的翻译起始不依赖于S1。通过大肠杆菌无细胞翻译系统（S-100提取物），已显示不含S1的核糖体对微型cro-mRNA的翻译。与微型cro-mRNA相反，没有蛋白质S1的30S亚基在三元起始复合物形成以及用MS2或fr噬菌体RNA和噬菌体fd的RNA蛋白III进行的无细胞翻译中均无活性。
