Lobiński Ryszard, Schaumlöffel Dirk, Szpunar Joanna
Equipe de Chimie Analytique Bio-Inorganique, CNRS UMR 5034, Hélioparc, 2, av. du Pr. Angot, F-64053 Pau, France.
Mass Spectrom Rev. 2006 Mar-Apr;25(2):255-89. doi: 10.1002/mas.20069.
A considerable momentum has recently been gained by in vitro and in vivo studies of interactions of trace elements in biomolecules due to advances in inductively coupled plasma mass spectrometry (ICP MS) used as a detector in chromatography and capillary and planar electrophoresis. The multi-isotopic (including non-metals such as S, P, or Se) detection capability, high sensitivity, tolerance to matrix, and large linearity range regardless of the chemical environment of an analyte make ICP MS a valuable complementary technique to electrospray MS and MALDI MS. This review covers different facets of the recent progress in metal speciation in biochemistry, including probing in vitro interactions between metals and biomolecules, detection, determination, and structural characterization of heteroatom-containing molecules in biological tissues, and protein monitoring and quantification via a heteroelement (S, Se, or P) signal. The application areas include environmental chemistry, plant and animal biochemistry, nutrition, and medicine.
由于电感耦合等离子体质谱（ICP MS）作为色谱、毛细管电泳和平面电泳中的检测器取得了进展，最近生物分子中微量元素相互作用的体外和体内研究获得了相当大的发展势头。多同位素（包括S、P或Se等非金属）检测能力、高灵敏度、对基质的耐受性以及无论分析物的化学环境如何都具有较大的线性范围，使得ICP MS成为电喷雾质谱和基质辅助激光解吸电离质谱的一种有价值的补充技术。本综述涵盖了生物化学中金属形态分析近期进展的不同方面，包括探究金属与生物分子之间的体外相互作用、生物组织中含杂原子分子的检测、测定和结构表征，以及通过杂元素（S、Se或P）信号进行蛋白质监测和定量。应用领域包括环境化学、植物和动物生物化学、营养和医学。
