在SiSo细胞上表达的受体结合癌抗原可在胃肠道癌转移淋巴结中检测到。
Receptor-binding cancer antigen expressed on SiSo cells can be detected in metastatic lymph nodes from gastrointestinal cancers.
作者信息
Leelawat Kawin, Engprasert Surang, Tujinda Supathip, Suthippintawong Cheepsumon, Enjoji Munechika, Nakashima Manabu, Watanabe Takeshi, Leardkamolkarn Vijittra
机构信息
Department of Surgery, Rajavithi Hospital, Rajavithi Road, Rajathevi, Bangkok 10400, Thailand.
出版信息
World J Gastroenterol. 2005 Oct 14;11(38):6014-7. doi: 10.3748/wjg.v11.i38.6014.
AIM
To investigate the expression of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) in metastatic lymph nodes from gastrointestinal cancer.
METHODS
Metastatic lymph nodes from gastrointestinal cancer were detected for RCAS1 by immunohistochemical staining and mRNA in situ hybridization.
RESULTS
A total of 102 metastatic lymph nodes from bile duct, gastric, colon and pancreatic cancer were investigated for RCAS1 expression. The immunoreactivity of RCAS1 was identified in 100% of metastatic lymph nodes. Both local and distant metastatic lymph nodes showed RCAS1 expression. On the contrary, specimens of non-cancerous lymph nodes were negative for RCAS1. The result of mRNA in situ hybridization was also confirmed by the finding of immunohistochemical staining. RCAS1 mRNA was detected in all tumor cells that metastasized to lymph nodes.
CONCLUSION
All metastatic lymph nodes express RCAS1 in tumor cells at both mRNA levels, and RCAS1 that should be used as a complementary factor for identification of metastatic lymph nodes from gastrointestinal cancers.
目的
研究SiSo细胞上表达的受体结合癌抗原（RCAS1）在胃肠道癌转移淋巴结中的表达情况。
方法
采用免疫组织化学染色和mRNA原位杂交法检测胃肠道癌转移淋巴结中的RCAS1。
结果
共对102个来自胆管癌、胃癌、结肠癌和胰腺癌的转移淋巴结进行了RCAS1表达检测。100%的转移淋巴结中均检测到RCAS1的免疫反应性。局部和远处转移淋巴结均有RCAS1表达。相反，非癌性淋巴结标本中RCAS1呈阴性。mRNA原位杂交结果也得到了免疫组织化学染色结果的证实。在所有转移至淋巴结的肿瘤细胞中均检测到RCAS1 mRNA。
结论
所有转移淋巴结中的肿瘤细胞在mRNA水平均表达RCAS1，RCAS1可作为鉴别胃肠道癌转移淋巴结的一个补充指标。
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