Wang Yun-Shan, Ma Xiao-Li, Qi Tong-Gang, Liu Xiang-Dong, Meng Yue-Sheng, Guan Guang-Ju
Jinan Central Hospital, 105# Jinan Jiefang Road, Jinan 250013, Shandong Province, China.
World J Gastroenterol. 2005 Oct 14;11(38):6053-5. doi: 10.3748/wjg.v11.i38.6053.
To study the function of alpha-fetoprotein (AFP) in SMMC-7721 hepatoma cells.
A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescence activated cell sorter (FACS).
The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.
Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.
研究甲胎蛋白（AFP）在SMMC - 7721肝癌细胞中的功能。
构建表达短发夹RNA的质粒pSilencer3.0 - H1 - afp，并使用脂质体2000将其转染至SMMC - 7721细胞中。通过实时逆转录聚合酶链反应（RT - PCR）和免疫测定法监测AFP的表达，采用MTT法和荧光激活细胞分选仪（FACS）检测其对SMMC - 7721细胞增殖和细胞死亡的影响。
表达AFP - siRNA的质粒显著下调AFP的表达（约34%），并抑制SMMC - 7721细胞增殖，但未诱导细胞凋亡。
下调AFP - siRNA可抑制SMMC - 7721细胞增殖，但不会导致细胞凋亡。
