Losi L, Benhattar J, Costa J
Institut Universitaire de Pathologie, Lausanne, Switzerland.
Eur J Cancer. 1992;28A(6-7):1115-20. doi: 10.1016/0959-8049(92)90468-h.
We have used a rapid, non-radioactive and sensitive method based on allele-specific amplification using the polymerase chain reaction for the identification of K-ras mutations in archival tissues of colorectal carcinomas. Our purpose was to determine whether or not K-ras mutation provides, when present, a tumour marker throughout the natural history of the disease. We have studied 35 patients who developed recurrent cancer. In 71% of these patients a ras mutation in codons 12 or 13 was observed in the primary tumour. For each of these cases an identical ras mutation was found in the DNA from the local or distant recurrence. In the 29% of cases where no ras mutation was observed in the primary tumour, no newly acquired ras mutation appeared in the recurrent tumour. The time interval between primary tumours and recurrences varied from 3 to 60 months. Our results indicate that K-ras mutation provides a stable tumour marker throughout the natural history of colorectal cancer.
我们采用了一种基于聚合酶链反应的等位基因特异性扩增的快速、非放射性且灵敏的方法，用于鉴定结直肠癌存档组织中的K-ras突变。我们的目的是确定K-ras突变在疾病自然史中（若存在）是否可作为一种肿瘤标志物。我们研究了35例发生复发性癌症的患者。在这些患者中，71%的原发性肿瘤中观察到密码子12或13处的ras突变。对于每一例，在局部或远处复发的DNA中发现了相同的ras突变。在29%的原发性肿瘤中未观察到ras突变的病例中，复发性肿瘤中未出现新获得的ras突变。原发性肿瘤与复发之间的时间间隔为3至60个月。我们的结果表明，K-ras突变在结直肠癌的整个自然史中提供了一种稳定的肿瘤标志物。
