韭菜（葱属植物葱）叶片中酰基辅酶A延长酶含量的评估。
Evaluation of the amount of acyl-CoA elongases in leek (Allium porrum L) leaves.
作者信息
Bessoule J J, Creach A, Lessire R, Cassagne C
机构信息
Institut de Biochimie Cellulaire et Neurochimie du CNRS, Université de Bordeaux, France.
出版信息
Biochim Biophys Acta. 1992 Jul 21;1117(1):78-82. doi: 10.1016/0304-4165(92)90165-q.
Polyclonal antibodies have been raised against the acyl-CoA elongase purified from leek epidermal cells. The antibodies recognize the fractions containing the elongating activity after DEAE or Ultrogel chromatography and their response with the other fractions is very low. The immune complex is immunoprecipitable with Protein A-Sepharose. 1% of the solubilized proteins from leek epidermis microsomes are immunoprecipitated. The immunoprecipitate contains an elongating activity which is 86 +/- 20-times that of the unbound fraction.
已经制备了针对从韭菜表皮细胞中纯化的酰基辅酶A延长酶的多克隆抗体。这些抗体能够识别经DEAE或Ultrogel柱层析后含有延长活性的组分，而它们与其他组分的反应非常低。免疫复合物可用蛋白A-琼脂糖进行免疫沉淀。从韭菜表皮微粒体中溶解的蛋白质有1%被免疫沉淀。免疫沉淀物含有一种延长活性，其活性是未结合组分的86±20倍。
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