Schneider F, Lessire R, Bessoule J J, Juguelin H, Cassagne C
Institut de Biochimie Cellulaire, CNRS, Bordeaux, France.
Biochim Biophys Acta. 1993 Nov 7;1152(2):243-52. doi: 10.1016/0005-2736(93)90255-x.
Cerulenin inhibits the elongation of stearoyl-CoA and eicosanoyl-CoA by microsomes from leek seedlings. The inhibition depends on the cerulenin concentration and affects the biosynthesis of docosanoic and tetracosanoic acids only slightly more than that of eicosanoic acid. A 30-min preincubation of the microsomes with cerulenin allows a quantitative inhibition of the elongation at 50 microM cerulenin (50% inhibition at 15 microM cerulenin). A kinetic study of the elongating activity in the presence or in the absence of the inhibitor suggests that the inhibition is non-competitive. Analysis of the products of the reaction suggests that 3-ketoacyl-CoA synthase is the target of cerulenin. A study of the partial reactions demonstrates that the inhibition affects almost exclusively the condensation step.
浅蓝菌素可抑制韭菜幼苗微粒体对硬脂酰辅酶A和二十碳酰辅酶A的延长作用。这种抑制作用取决于浅蓝菌素的浓度，对二十二碳酸和二十四碳酸生物合成的影响仅比对二十碳酸生物合成的影响略大一点。微粒体与浅蓝菌素预温育30分钟后，在50微摩尔浅蓝菌素时可实现对延长作用的定量抑制（在15微摩尔浅蓝菌素时抑制率达50%）。对有或没有抑制剂存在时延长活性的动力学研究表明，这种抑制作用是非竞争性的。对反应产物的分析表明，3-酮脂酰辅酶A合酶是浅蓝菌素的作用靶点。对部分反应的研究表明，这种抑制几乎只影响缩合步骤。
