一种在大肠杆菌细胞中生产人碱性成纤维细胞生长因子的快速方法。
A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells.
作者信息
Ke Y, Wilkinson M C, Fernig D G, Smith J A, Rudland P S, Barraclough R
机构信息
Department of Biochemistry, University of Liverpool, UK.
出版信息
Biochim Biophys Acta. 1992 Jul 15;1131(3):307-10. doi: 10.1016/0167-4781(92)90029-y.
PMID:1627646
Abstract
Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.
摘要
利用一种仅需简单DNA操作的快速方法，人碱性成纤维细胞生长因子已在大肠杆菌细胞中表达，表达水平为每升培养物2 - 3毫克。重组产物与来自牛垂体的天然碱性成纤维细胞生长因子具有相同的效力。
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