Bingle C D, Kelly F, Epstein O, Srai S K
Department of Protein and Molecular Biology, Royal Free Hospital School of Medicine, London UK.
Biochim Biophys Acta. 1992 Jul 7;1139(3):217-21. doi: 10.1016/0925-4439(92)90137-c.
We have previously shown that mRNA for caeruloplasmin, the serum copper-binding protein, is not expressed in guinea-pig liver prior to birth and expression increases rapidly following parturition. To further study the regulation of caeruloplasmin in neonatal animals we have used 3-day preterm guinea pigs, delivered by caesarean section, to investigate mRNA expression in liver and lung. Within 12 h of premature birth, hepatic caeruloplasmin mRNA levels are significantly increased and remain elevated by 72 h. This induction of expression is accompanied by an increase in circulating levels of holoprotein. Even greater induction of caeruloplasmin mRNA was observed in premature animals maintained in 95% oxygen for 72 h, confirming an acute-phase response in prematurity. Studies of lung RNA showed that caeruloplasmin mRNA is expressed throughout development, with highest levels observed in adult lung. In the premature animals levels were significantly elevated 12 h after delivery, but then fell by 72 h to below those seen in normal term lung. Hyperoxia did not influence the pulmonary mRNA levels.
我们之前已经表明，血清铜结合蛋白铜蓝蛋白的mRNA在豚鼠出生前的肝脏中不表达，而在分娩后迅速增加。为了进一步研究新生动物中铜蓝蛋白的调控，我们使用了剖宫产出生的早产3天的豚鼠，来研究肝脏和肺中的mRNA表达。早产12小时内，肝脏铜蓝蛋白mRNA水平显著增加，并在72小时内保持升高。这种表达的诱导伴随着全蛋白循环水平的增加。在95%氧气环境中维持72小时的早产动物中观察到铜蓝蛋白mRNA的诱导作用更强，证实了早产中的急性期反应。肺RNA研究表明，铜蓝蛋白mRNA在整个发育过程中都有表达，在成年肺中观察到的水平最高。在早产动物中，分娩后12小时水平显著升高，但到72小时时降至正常足月肺中的水平以下。高氧不影响肺mRNA水平。
