Benes Milan J, Horák Daniel, Svec Frantisek
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Sep Sci. 2005 Oct;28(15):1855-75. doi: 10.1002/jssc.200500186.
This review summarizes the preparation and application of chromatographic separation media based on methacrylate monomers with a major focus on highly crosslinked macroporous beads prepared from 2-hydroxyethyl methacrylate and glycidyl methacrylate, respectively. The effects of process variables such as composition of the polymerization mixture that includes monomers, porogenic solvents, and free radical initiator, suspension stabilizer, reaction temperature, and stirring are detailed for both classical and templated suspension polymerization. In addition, specific features of the preparation of monodisperse beads are also discussed. The performance of methacrylate-based separation media is demonstrated on numerous separations in a variety of chromatographic modes.
本综述总结了基于甲基丙烯酸酯单体的色谱分离介质的制备及应用，主要聚焦于分别由甲基丙烯酸2-羟乙酯和甲基丙烯酸缩水甘油酯制备的高度交联大孔微球。详细阐述了经典悬浮聚合和模板悬浮聚合过程中工艺变量的影响，如包含单体、致孔剂、自由基引发剂、悬浮稳定剂、反应温度和搅拌的聚合混合物的组成。此外，还讨论了单分散微球制备的具体特点。基于甲基丙烯酸酯的分离介质在多种色谱模式下的众多分离中展现出了性能。
