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微芯片上毛细管电泳的应用。

Applications of capillary electrophoresis on microchip.

作者信息

Dolnik Vladislav, Liu Shaorong

机构信息

Alcor BioSeparations, Santa Clara, CA 95051, USA.

出版信息

J Sep Sci. 2005 Oct;28(15):1994-2009. doi: 10.1002/jssc.200500243.

Abstract

CE on microchip is an emerging separation technique that has attracted wide attention and gained considerable popularity. Because of miniaturization of the separation format, CE on chip typically offers shorter analysis time and lower reagent consumption with potential development of portable analytical instrumentation. This review with 143 references is focused on proteins and peptides analysis, DNA separation including fragment sizing, genotyping, mutation detection and sequencing, and also the analysis of low-molecular-weight compounds, namely explosive residues and warfare agents, pharmaceuticals and drugs of abuse, and various small molecules in body fluids.

摘要

芯片上的毛细管电泳是一种新兴的分离技术,已引起广泛关注并颇受欢迎。由于分离形式的小型化,芯片上的毛细管电泳通常具有更短的分析时间和更低的试剂消耗,并且具备便携式分析仪器的潜在发展前景。这篇综述引用了143篇参考文献,重点关注蛋白质和肽分析、DNA分离(包括片段大小测定、基因分型、突变检测和测序),以及低分子量化合物分析,即爆炸物残留和战争毒剂、药物和滥用药物,以及体液中的各种小分子。

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