Nuchtavorn Nantana, Suntornsuk Worapot, Lunte Susan M, Suntornsuk Leena
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayudhaya Rd., Rajathevee, Bangkok 10400, Thailand; School of Physical Sciences and Australian Centre for Research on Separation Science (ACROSS), University of Tasmania, Private Bag 75, Hobart 7001, Australia.
Department of Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd., Bangmod, Thrungkru, Bangkok 10140, Thailand.
J Pharm Biomed Anal. 2015 Sep 10;113:72-96. doi: 10.1016/j.jpba.2015.03.002. Epub 2015 Mar 20.
Many separation methods have been developed for biomedical analysis, including chromatographic (e.g. high performance liquid chromatography (HPLC) and gas chromatography (GC)) and electrophoretic methods (e.g. gel electrophoresis and capillary electrophoresis (CE)). Among these techniques, CE provides advantages in terms of high separation efficiency, simplicity, low sample and solvent volume consumption, short analysis time and applicability to a wide range of biomedically important substances. Microchip electrophoresis (ME) is a miniaturized platform of CE and is now considered as a simpler and more convenient alternative, which has demonstrated potential in analytical chemistry. High-throughput, cost-effective and portable analysis systems can be developed using ME. The current review describes different separation modes and detectors that have been employed in ME to analyze various classes of biomedical analytes (e.g. pharmaceuticals and related substances, nucleic acids, amino acids, peptides, proteins, antibodies and antigens, carbohydrates, cells, cell components and lysates). Recent applications (during 2010-2014) in these areas are presented in tables and some significant findings are highlighted.
已开发出多种用于生物医学分析的分离方法，包括色谱法（如高效液相色谱法（HPLC）和气相色谱法（GC））以及电泳法（如凝胶电泳和毛细管电泳（CE））。在这些技术中，CE在分离效率高、操作简单、样品和溶剂量消耗低、分析时间短以及适用于多种具有生物医学重要性的物质等方面具有优势。微芯片电泳（ME）是CE的小型化平台，现在被认为是一种更简单、更便捷的替代方法，在分析化学中已展现出潜力。使用ME可开发高通量、经济高效且便携的分析系统。本综述描述了在ME中用于分析各类生物医学分析物（如药物及相关物质、核酸、氨基酸、肽、蛋白质、抗体和抗原、碳水化合物、细胞、细胞成分和裂解物）的不同分离模式和检测器。表格中列出了2010年至2014年期间在这些领域的最新应用，并突出了一些重要发现。
