Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Electrophoresis. 2014 Jan;35(1):69-95. doi: 10.1002/elps.201300331. Epub 2013 Nov 20.
The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.
本综述全面介绍了近年来毛细管和微芯片电泳分离方法（区带电泳、等电聚焦、等速电泳、亲和电泳、胶束电动色谱和电色谱）在分析、分离、纯化以及肽的物理化学和生化特性表征方面的最新进展和应用。本文介绍了在研究肽的电动迁移特性、分析方法学方面的进展，包括样品预分离、预浓缩和衍生化、吸附抑制和EOF 控制，以及肽的检测。报道了在特定 CE 和 CEC 模式方面的新发展，并描述了它们在肽分析中的几种应用类型：常规定性和定量分析、复杂（生物）基质中的测定、化学和酶反应以及物理变化的监测、氨基酸、序列和手性分析以及蛋白质的肽图分析。展示了一些微制备肽分离，并证明了 CE 和 CEC 技术能够提供有关肽的物理化学特性。
