液相色谱 - 质谱分析证明二氢血根碱是血根碱在大鼠体内转化的首要代谢产物。
A liquid chromatographic-mass spectrometric evidence of dihydrosanguinarine as a first metabolite of sanguinarine transformation in rat.
作者信息
Psotová Jitka, Klejdus Borivoj, Vecera Rostislav, Kosina Pavel, Kubán Vlastimil, Vicar Jaroslav, Simánek Vilím, Ulrichová Jitka
机构信息
Institute of Medical Chemistry and Biochemistry, Faculty of Medicine, Palacký University, Olomouc, Czech Republic.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 2;830(1):165-72. doi: 10.1016/j.jchromb.2005.10.030. Epub 2005 Nov 8.
Adult rats were orally administered with a single dose of sanguinarine (10 mg SA per 1 kg body weight) in 1.0 ml water. In the plasma and the liver, dihydrosanguinarine (DHSA) was identified as a SA metabolite by high performance liquid chromatography-electrospray ionization mass spectrometry (HPLC/ESI-MS). Significantly higher levels of DHSA were found in both the plasma and the liver in comparison with those of SA. SA and DHSA were not detected in the urine. The formation of DHSA might be the first step of SA detoxification in the organism and its subsequent elimination in phase II reactions. Benz[c]acridine (BCA), in the literature cited SA metabolite, was found neither in urine nor in plasma and liver.
成年大鼠经口给予单剂量的血根碱（每1千克体重10毫克血根碱，溶于1.0毫升水中）。通过高效液相色谱-电喷雾电离质谱法（HPLC/ESI-MS）在血浆和肝脏中鉴定出二氢血根碱（DHSA）为血根碱的代谢产物。与血根碱相比，在血浆和肝脏中均发现二氢血根碱的水平显著更高。在尿液中未检测到血根碱和二氢血根碱。二氢血根碱的形成可能是生物体中血根碱解毒的第一步，随后在II相反应中被消除。文献中提及的血根碱代谢产物苯并[c]吖啶（BCA）在尿液、血浆和肝脏中均未被发现。
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