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高效液相色谱-质谱联用技术在中药分析中的应用。

Applications of HPLC/MS in the analysis of traditional Chinese medicines.

作者信息

Li Miao, Hou Xiao-Fang, Zhang Jie, Wang Si-Cen, Fu Qiang, He Lang-Chong

机构信息

School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

J Pharm Anal. 2011 May;1(2):81-91. doi: 10.1016/S2095-1779(11)70015-6. Epub 2012 Jan 30.

DOI:10.1016/S2095-1779(11)70015-6
PMID:29403684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5760786/
Abstract

In China, traditional Chinese medicines (TCMs) have been used in clinical applications for thousands of years. The successful hyphenation of high-Performance liquid chromatography (HPLC) and mass spectrometry (MS) has been applied widely in TCMs and biological samples analysis. Undoubtedly, HPLC/MS technique has facilitated the understanding of the treatment mechanism of TCMs. We reviewed more than 350 published papers within the last 5 years on HPLC/MS in the analysis of TCMs. The present review focused on the applications of HPLC/MS in the component analysis, metabolites analysis, and pharmacokinetics of TCMs etc. 50% of the literature is related to the component analysis of TCMs, which show that this field is the most populär type of research. In the metabolites analysis, HPLC coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry has been demonstrated to be the powerful tool for the characterization of structural features and fragmentation behavior patterns. This paper presented a brief overview of the applications of HPLC/MS in the analysis of TCMs. HPLC/MS in the fingerprint analysis is reviewed elsewhere.

摘要

在中国，中药已在临床应用中使用了数千年。高效液相色谱（HPLC）与质谱（MS）的成功联用已广泛应用于中药和生物样品分析。毫无疑问，HPLC/MS技术促进了对中药治疗机制的理解。我们查阅了过去5年里发表的350多篇关于HPLC/MS在中药分析中的论文。本综述聚焦于HPLC/MS在中药成分分析、代谢产物分析和药代动力学等方面的应用。50%的文献与中药成分分析相关，这表明该领域是最热门的研究类型。在代谢产物分析中，HPLC与电喷雾电离四极杆飞行时间串联质谱联用已被证明是表征结构特征和碎片行为模式的有力工具。本文简要概述了HPLC/MS在中药分析中的应用。HPLC/MS在指纹图谱分析方面的内容在其他地方已有综述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/6cd57405ae6f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/21eaa1f6098c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/75296cf5967e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/785a22960902/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/6cd57405ae6f/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/21eaa1f6098c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/75296cf5967e/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/785a22960902/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53ce/5760786/6cd57405ae6f/gr4.jpg

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