Jain P K, Jain P C
Department of Applied Microbiology and Biotechnology, Dr. H.S. Gour Vishwavidyalaya SAGAR - 470 003 (M.P.), India.
Hindustan Antibiot Bull. 2003;45-46(1-4):5-10.
A total of 287 actinomycetes were isolated from 79 samples collected from five different habitats i.e., cultivated field soil (CFS), garden soil (GS), compost (CM), decaying organic matter (DOM) and stored agricultural products (SAP) of different localities of Sagar, Madhya Pradesh (23 degrees 50 degrees N latitude and 78 degrees 40 degrees E longitude). These were screened for antagonistic activity against Candida albicans, Aspergillus niger, Microsporum gypseun and Trichophyton sp. using 'Cross streak method'. Out of these, a total of 166 isolates were found antagonistic to Candida albicans, while 164, 134 and 132 actinomycetes showed antagonistic properties against A. niger, M. gypseum and Trichophyton sp., repectively. A total of 17 isolates showed very strong anticandidal activity causing total inhibition in the growth of C. albicans and hence, distribution of isolated test actinomycetes in different habitats and the cultural and antagonistic properties of selected 17 promising strains are reported here.
从印度中央邦萨加尔不同地点的五个不同栖息地采集的79个样本中,共分离出287株放线菌,这些栖息地包括耕地土壤(CFS)、花园土壤(GS)、堆肥(CM)、腐烂有机物(DOM)和储存农产品(SAP)(北纬23度50分,东经78度40分)。使用“交叉划线法”对这些放线菌进行筛选,检测其对白色念珠菌、黑曲霉、石膏样小孢子菌和毛癣菌的拮抗活性。其中,共有166株分离菌对白色念珠菌具有拮抗作用,而分别有164株、134株和132株放线菌对黑曲霉、石膏样小孢子菌和毛癣菌表现出拮抗特性。共有17株分离菌表现出非常强的抗念珠菌活性,能完全抑制白色念珠菌的生长,因此,本文报道了分离得到的测试放线菌在不同栖息地的分布情况以及所选17株有潜力菌株的培养特性和拮抗特性。