Ellaiah P, Raju K V V S N Bapi, Adinarayana K, Adinarayana G, Saisha V, Madhavi S, Premkumar J
Pharmaceutical Biotechnology Division, Department of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530 003, India.
Hindustan Antibiot Bull. 2002 Feb-Nov;44(1-4):8-16.
Sediment samples from Krishna River at Nagayalanka of Andhra Pradesh, India were investigated as a source of actinomycetes to screen for the production of novel bioactive compounds. During our investigation on fresh water actinomycetes from 5 different river sediment samples, a total of 80 actinomycetes were isolated. Out of these 80 isolates, 30 isolates which showed distinct macromorphological characteristics were selected. The antimicrobial and enzymatic activities were studied for all the 30 isolates. The preliminary study for antimicrobial activity by cross streak method indicated that 16 isolates (53.3%) have excellent antagonistic properties. All these 16 isolates were subjected to detailed submerged fermentation studies. It was observed that 12 isolates (40.0%) exhibited antibacterial activity, 9 isolates (30.0%) showed antifungal activity while 5 isolates (16.6%) showed both antibacterial and antifungal activities. All the 30 isolates were also subjected for the determination of enzymatic activities 25 isolates (83.3%) exhibited amylolytic activity while 27 isolates (90.0%) showed proteolytic activity. Among these isolates, six promising isolates were selected for detailed morphological, cultural, physiological and biochemical studies. It was established that these isolates belong to the Streptomyces genus by virtue of their cell wall composition pattern and were identified as strains of different Streptomyces species like S. rochei, S. alanosinicus, S. erumpens, S. griseoplanus, S. gancidicus and S. nigrogriseolus.
对来自印度安得拉邦纳加亚兰卡的克里希纳河的沉积物样本进行了研究，以寻找放线菌来源，筛选新型生物活性化合物的产生。在对5个不同河流沉积物样本中的淡水放线菌进行调查期间，共分离出80株放线菌。在这80株分离物中，选择了30株具有明显宏观形态特征的分离物。对所有30株分离物进行了抗菌和酶活性研究。通过交叉划线法进行的抗菌活性初步研究表明，16株分离物（53.3%）具有优异的拮抗特性。所有这16株分离物都进行了详细的深层发酵研究。观察到12株分离物（40.0%）表现出抗菌活性，9株分离物（30.0%）表现出抗真菌活性，而5株分离物（16.6%）表现出抗菌和抗真菌活性。所有30株分离物也进行了酶活性测定，25株分离物（83.3%）表现出淀粉酶活性，27株分离物（90.0%）表现出蛋白水解活性。在这些分离物中，选择了6株有前景的分离物进行详细的形态、培养、生理和生化研究。根据它们的细胞壁组成模式确定这些分离物属于链霉菌属，并被鉴定为不同链霉菌种的菌株，如罗氏链霉菌、阿拉诺西链霉菌、突链霉菌、灰褐链霉菌、甘西链霉菌和黑灰链霉菌。
