死后人类大脑中磷脂酰肌醇特异性磷脂酶C活性：阿尔茨海默病无改变。

Phosphatidylinositol-specific phospholipase C activity in the postmortem human brain: no alteration in Alzheimer's disease.

作者信息

Shimohama S, Fujimoto S, Taniguchi T, Kimura J

机构信息

Department of Neurology, Faculty of Medicine, Kyoto University, Japan.

出版信息

Brain Res. 1992 May 8;579(2):347-9. doi: 10.1016/0006-8993(92)90073-i.

DOI:10.1016/0006-8993(92)90073-i

PMID:1628221

Abstract

The activity of phospholipase C (PLC) which hydrolyzes exogenous phosphatidylinositol (PI), was investigated in samples prepared from postmortem normal human brains and Alzheimer disease brains. The enzyme activity did not change significantly after rat brains were left for 24 h at room temperature. The PI-specific PLC activity in the Alzheimer cytosolic and particulate fractions was not significantly different from that in the control fractions. The PI-specific PLC activity as a function of the free Ca2+ concentration was also similar between control and Alzheimer brains. These results suggest that the PI-specific PLC activity is not altered in Alzheimer's disease.

摘要

研究了从正常人死后大脑和阿尔茨海默病大脑制备的样本中，水解外源性磷脂酰肌醇（PI）的磷脂酶C（PLC）的活性。大鼠大脑在室温下放置24小时后，酶活性没有显著变化。阿尔茨海默病细胞质和微粒体部分的PI特异性PLC活性与对照部分没有显著差异。对照大脑和阿尔茨海默病大脑中，作为游离Ca2+浓度函数的PI特异性PLC活性也相似。这些结果表明，PI特异性PLC活性在阿尔茨海默病中没有改变。