Yuvienco Jose Mari S, Dizon Ma Emily C, Kleinfeld Alan, Anwar Mujahid, Hiatt Mark, Hegyi Thomas
Division of Neonatology, Department of Pediatrics, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA.
Am J Perinatol. 2005 Nov;22(8):429-36. doi: 10.1055/s-2005-916333.
Increased levels of unbound Free Fatty acid (FFAu) have been found in adults undergoing coronary angioplasty as a result of acute hypoxia-ischemia. We hypohesized that infants suffering from a 1-minute Apgar score of less than 5 will demonstrate elevated FFAu levels in the cord blood. One hundred ninety-nine infants between 25 and 41 weeks gestational age were enrolled in the study. Infants with an Apgar score of less than 5 at 1 minute served as the study group. Blood samples were collected from the umbilical cord and serum FFAu levels were measured with the fluorescent probe acrylodan-derivatized intestinal fatty acid binding protein. The low Apgar score group (n=32, birthweight 3153+/-780 g, gestational age 37.9+/-3.1 weeks) and normal Apgar score group (n=167, birthweight 3067+/-847 g, gestational age 37.5+/-3.5 weeks) were significantly different with respect to Apgar score at 1 minute (3.0+/-1.2 versus 8.4+/-1.1), Apgar score at 5 minutes (6.9+/-versus 8.9+/-0.5), cord pH (7.16+/-0.12 versus 7.28+/-0.07), and in the frequency of meconium passage (40.6% versus 14.9%). Cord FFAu levels were 4.4+/-1.7 versus 3.2+/-1.2 nM (p<0.001), respectively. Cord FFAu correlated inversely with Apgar score at 1 minute (r=-0.31, p<0.05) and with cord pH (r=-0.12, p<0.05), but not with birthweight or gestational age. In infants with low 1-minute Apgar scores, cord free fatty acid levels were significantly elevated compared with those from controls.
在因急性缺氧缺血而接受冠状动脉血管成形术的成年人中，已发现游离脂肪酸（FFAu）水平升高。我们推测，1分钟阿氏评分低于5分的婴儿脐带血中FFAu水平会升高。199名孕龄在25至41周之间的婴儿被纳入该研究。1分钟时阿氏评分低于5分的婴儿作为研究组。从脐带采集血样，并用荧光探针丙烯罗丹衍生化的肠脂肪酸结合蛋白测量血清FFAu水平。低阿氏评分组（n = 32，出生体重3153±780 g，孕龄37.9±3.1周）和正常阿氏评分组（n = 167，出生体重3067±847 g，孕龄37.5±3.5周）在1分钟时的阿氏评分（3.0±1.2对8.4±1.1）、5分钟时的阿氏评分（6.9±对8.9±0.5）、脐带血pH值（7.16±0.12对7.28±0.07）以及胎粪排出频率（40.6%对14.9%）方面存在显著差异。脐带FFAu水平分别为4.4±1.7对3.2±1.2 nM（p<0.001）。脐带FFAu与1分钟时的阿氏评分呈负相关（r = -0.31，p<0.05），与脐带血pH值呈负相关（r = -0.12，p<0.05），但与出生体重或孕龄无关。在1分钟阿氏评分低的婴儿中，脐带游离脂肪酸水平与对照组相比显著升高。
