Weinstein I B, Orenstein J M, Gebert R, Kaighn M E, Stadler U C
Cancer Res. 1975 Jan;35(1):253-63.
Epithelial cultures established from adult rat liver and from rat hepatomas induced in vivo with aromatic amine carcinogens have been compared by light and electron microscopy and by growth properties in liquid medium and in agar. The morphology and growth patterns of all of these cultures indicate that they have characteristics of epithelial rather than fibroblast cells. The criteria generally used to score for transformation of fibroblasts were not satisfactory for distinguishing normal epithelial cells from hepatoma cells in culture. Growth in agar, however, provides a simple and objective method of scoring for transformed epithelial cells, because only the tumorigenic cells grow in agar. Since none of the normal cultures had hydrocortisone-inducible tryosine aminotransferase, we lack definitive evidence that they are derived from liver parenchymal cells. The outstanding feature in the ultrastructure of the hepatoma cells in culture was the presence of type A and C viral particles. Whereas five hepatoma cultures and a spontaneously transformed normal liver cell line were positive for these particles, five independently isolated cell cultures from normal adult rat liver were negative. Evidence is presented that the viral particles seen in hepatoma cultures are due to activation of latent viruses rather than to in vitro contamination. The possible significance of these particles in hepatocarcinogenesis is discussed.
通过光学显微镜和电子显微镜以及在液体培养基和琼脂中的生长特性，对从成年大鼠肝脏以及用芳香胺致癌物在体内诱导产生的大鼠肝癌建立的上皮细胞培养物进行了比较。所有这些培养物的形态和生长模式表明它们具有上皮细胞而非成纤维细胞的特征。通常用于评估成纤维细胞转化的标准，对于区分培养中的正常上皮细胞和肝癌细胞并不令人满意。然而，在琼脂中生长提供了一种简单而客观的评估转化上皮细胞的方法，因为只有致瘤细胞能在琼脂中生长。由于所有正常培养物都没有氢化可的松诱导的酪氨酸转氨酶，我们缺乏确凿证据证明它们源自肝实质细胞。培养的肝癌细胞超微结构的突出特征是存在A 型和C 型病毒颗粒。虽然五种肝癌培养物和一个自发转化的正常肝细胞系对这些颗粒呈阳性，但从正常成年大鼠肝脏独立分离的五种细胞培养物呈阴性。有证据表明，在肝癌培养物中看到的病毒颗粒是由于潜伏病毒的激活而非体外污染。讨论了这些颗粒在肝癌发生中的可能意义。
