San R H, Laspia M F, Soiefer A I, Maslansky C J, Rice J M, Williams G M
Cancer Res. 1979 Mar;39(3):1026-34.
Continuous epithelial-like cell lines derived from normal adult rat liver and hepatocarcinomas were evaluated for their growth in soft agar and five properties of the cell membrane as markers for neoplastic transformation. A correlation of these properties was made to the tumorigenicity of the lines in nude mice. Growth in soft agar was a specific and sensitive marker, whereas the data on uptake of 2-deoxy-D-glucose were consistent, with high uptake being a specific but clearly not a sensitive marker. Agglutination and hemadsorption mediated by concanavalin A, multinucleation in the presence of cytochalasin B, and the cell membrane activity of adenosine triphosphatase did not correlate with tumorigenicity of the other markers for transformation. In addition, it is shown that Mycoplasma infection does not alter any of these properties but that infection can be eliminated by passage of cells through nude mice.
对源自正常成年大鼠肝脏和肝癌的连续上皮样细胞系进行了评估，检测其在软琼脂中的生长情况以及作为肿瘤转化标志物的细胞膜的五种特性。将这些特性与这些细胞系在裸鼠中的致瘤性进行了关联。在软琼脂中生长是一种特异性且敏感的标志物，而关于2-脱氧-D-葡萄糖摄取的数据是一致的，高摄取是一种特异性但显然不敏感的标志物。伴刀豆球蛋白A介导的凝集和血细胞吸附、细胞松弛素B存在下的多核化以及三磷酸腺苷酶的细胞膜活性与其他转化标志物的致瘤性不相关。此外，研究表明支原体感染不会改变这些特性中的任何一种，但通过将细胞传代至裸鼠可消除感染。
