Bourette R P, Royet J, Mouchiroud G, Schmitt E, Blanchet J P
Centre de Génétique Moléculaire et Cellulaire, UMR CNRS no. 106, Université Claude Bernard Lyon I, Villeurbanne, France.
Exp Hematol. 1992 Aug;20(7):868-73.
Murine interleukin 9 (mIL-9) is a T-cell-derived growth factor that stimulates erythroid burst-forming units (BFU-E) from murine bone marrow. We further investigated this activity using enriched mouse bone marrow progenitors and the multipotent interleukin 3 (IL-3)-dependent FDCP-Mix cell line. We report here that mIL-9 stimulates erythroid burst formation of total bone marrow cells and accessory cell-depleted bone marrow cells, even in serum-free cultures. On the other hand, we observed that although mIL-9 could not support proliferation of FDCP-Mix cells, it favors erythroid differentiation of these cells in the presence of both IL-3 and erythropoietin. These results strongly suggest that mIL-9 acts directly on mouse erythroid progenitor cells.
小鼠白细胞介素9（mIL-9）是一种由T细胞产生的生长因子，可刺激小鼠骨髓中的红系爆式集落形成单位（BFU-E）。我们使用富集的小鼠骨髓祖细胞和多能白细胞介素3（IL-3）依赖性FDCP-Mix细胞系进一步研究了这种活性。我们在此报告，即使在无血清培养中，mIL-9也能刺激全骨髓细胞和去除辅助细胞的骨髓细胞形成红系爆式集落。另一方面，我们观察到，尽管mIL-9不能支持FDCP-Mix细胞的增殖，但在IL-3和促红细胞生成素存在的情况下，它有利于这些细胞的红系分化。这些结果有力地表明，mIL-9直接作用于小鼠红系祖细胞。
