Rodriguez M H, Arnaud S, Blanchet J P
Centre de Génétique Moléculaire et Cellulaire, CNRS UMR 106, Université Claude Bernard Lyon I, Villeurbanne, France.
Exp Hematol. 1995 Jun;23(6):545-50.
The effect of interleukin-11 (IL-11) on cultures of bone marrow cells was investigated. We found that IL-11 increased, in a dose-dependent manner, the number of bursts in the presence of Epo in murine or human cells cultures. This effect was also observed in cultures of murine cells established in serum-free conditions as well as in cultures of CD34+ enriched human cells in serum-containing (but not serum-free) cultures. A linear relationship between the number of bursts and the plated cell number was observed with murine bone marrow cells or non-adherent mononuclear cells (NA-MNC) human bone marrow cells. Moreover, the effect of IL-11 was not abrogated when either anti-stem cell factor-receptor (anti-SCF-R), anti-IL-3, or anti-granulocyte/macrophage colony-stimulating factor (GM-CSF), three cytokines known to greatly synergize with IL-11, was added to cultures. These results lead us to conclude that IL-11 directly stimulates the proliferation of murine and human burst-forming units-erythroid (BFU-E).
研究了白细胞介素-11（IL-11）对骨髓细胞培养的影响。我们发现，在小鼠或人类细胞培养中，IL-11在有促红细胞生成素（Epo）存在的情况下，以剂量依赖的方式增加爆式集落形成单位（BFU）的数量。在无血清条件下建立的小鼠细胞培养物以及含血清（而非无血清）培养的富集人CD34+细胞培养物中也观察到了这种效应。在小鼠骨髓细胞或非贴壁单核细胞（NA-MNC）人骨髓细胞中，观察到爆式集落形成单位数量与接种细胞数量之间存在线性关系。此外，当向培养物中添加抗干细胞因子受体（抗SCF-R）、抗IL-3或抗粒细胞/巨噬细胞集落刺激因子（GM-CSF）这三种已知与IL-11有显著协同作用的细胞因子时，IL-11的效应并未消除。这些结果使我们得出结论，IL-11直接刺激小鼠和人类红系爆式集落形成单位（BFU-E）的增殖。
