Anderson J N, Peck E J, Clark J H
Endocrinology. 1975 Jan;96(1):160-7. doi: 10.1210/endo-96-1-160.
The relationship between estrogen receptor(R) binding by uterine nuclei and uterotrophic responses was examined. Immature rats received a single injection of estradiol (E2) or estriol (E3) and the following parameters were measured: accumulation and retention of the estrogen receptor by the nuceus of uterine cells; incorporation of 14C-glucose into CO2 lipid, protein and RNA; RNA polymerase activity; water imbibition and increased dry weight. E2 and E3 were of equal potency with regard to the rapid accumulation of R by the nucleus but differed with respect to long term retention of R. The concentrations of nuclear RE2 and RE3 complexes were equivalent between 1 and 3 hr after estrogen injection; however, by 6 hr RE2 remained significantly elevated while RE3 levels had fallen to control values. E2 and E3 were also of equal potency with respect to the stimulation of enhanced glucose utilization, water imbibition, the incorporation of 14C-glucose into lipid, protein and RNA 3 hours following an injection of the hormone. Likewise the activity of RNA polymerase was equally stimulated by E2 and E3 3 hr after injection. Thus all early uterotropic responses (0-3 hrs) that were measured were equally stimulated by E2 and E3. However, E3 failed to stimulate true uterine growth (increase dry weight 24 hr after injection), whereas E2 produced a significant stimulation of true uterine growth. These data suggest that the RE complex is capable of stimulating early uterotrohic events regardless of which estrogen is present; however, in order to produce true uterine growth the RE complex must be retained in the nucleus for long periods of time. This proposal was tested by the administration of repetitive injections of E3. This treatment resulted in an increase in dry weight that was equivalent to the growth that was produced by repetitive injections of E2. These results demonstrate that E2 and E3 elicit early uterotrophic responses with equal facility following a single injection but that only E2 causes true uterine growth. The ability of E2 to stimulate true uterine growth appears to be related to the time of residence of the RE complex in the nucleus.
研究了子宫细胞核雌激素受体（R）结合与子宫营养反应之间的关系。给未成熟大鼠单次注射雌二醇（E2）或雌三醇（E3），并测量以下参数：子宫细胞核雌激素受体的积累和保留；14C-葡萄糖掺入二氧化碳、脂质、蛋白质和RNA；RNA聚合酶活性；吸水和干重增加。就细胞核快速积累R而言，E2和E3效力相当，但在R的长期保留方面有所不同。雌激素注射后1至3小时，核RE2和RE3复合物的浓度相当；然而，到6小时时，RE2仍显著升高，而RE3水平已降至对照值。在注射激素3小时后，E2和E3在刺激葡萄糖利用增强、吸水、14C-葡萄糖掺入脂质、蛋白质和RNA方面的效力也相当。同样，注射后3小时，E2和E3对RNA聚合酶活性的刺激作用相同。因此，所测量的所有早期子宫营养反应（0至3小时）均受到E2和E3的同等刺激。然而，E3未能刺激子宫真正生长（注射后24小时干重增加），而E2对子宫真正生长有显著刺激作用。这些数据表明，无论存在哪种雌激素，RE复合物都能够刺激早期子宫营养事件；然而，为了使子宫真正生长，RE复合物必须长时间保留在细胞核中。通过重复注射E3对这一假设进行了验证。这种处理导致干重增加，与重复注射E2所产生的生长相当。这些结果表明，单次注射后，E2和E3引发早期子宫营养反应的能力相同，但只有E2会导致子宫真正生长。E2刺激子宫真正生长的能力似乎与RE复合物在细胞核中的停留时间有关。
