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利用STE11激酶的显性等位基因和STE7激酶的多重磷酸化揭示酵母信息素反应途径中各组分的作用顺序。

Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase.

作者信息

Cairns B R, Ramer S W, Kornberg R D

机构信息

Department of Cell Biology, Stanford University School of Medicine, California 94305.

出版信息

Genes Dev. 1992 Jul;6(7):1305-18. doi: 10.1101/gad.6.7.1305.

Abstract

The signal transduction pathway that mediates the response of haploid yeast cells to peptide mating pheromones involves several components including the protein kinases STE7 and STE11. We have isolated and characterized a dominant allele of the STE11 gene and have demonstrated that expression of an amino-terminally truncated form of STE11 protein causes constitutive activation of the mating pathway. Expression of this dominant STE11 allele also restored mating ability to certain sterile strains. In conjunction with the results of others, our epistasis results establish the following order of action of pathway components: STE2, GPA1(SCG1), STE4, STE5, STE11, STE7, STE12. Transduction of the signal from STE11 to STE7 may involve phosphorylation because STE7 displays several phosphorylation forms, and STE7 is multiply phosphorylated in response to either pheromone or coexpression of dominant STE11 protein. Further signal propagation appears to require STE7 protein kinase activity, because a catalytically impaired STE7 mutant is defective in the mating response.

摘要

介导单倍体酵母细胞对肽类交配信息素作出反应的信号转导途径涉及多个组分,包括蛋白激酶STE7和STE11。我们已分离并鉴定了STE11基因的一个显性等位基因,并证明氨基末端截短形式的STE11蛋白的表达会导致交配途径的组成型激活。这种显性STE11等位基因的表达也恢复了某些不育菌株的交配能力。结合其他研究结果,我们的上位性分析结果确定了途径组分的以下作用顺序:STE2、GPA1(SCG1)、STE4、STE5、STE11、STE7、STE12。信号从STE11转导至STE7可能涉及磷酸化,因为STE7呈现多种磷酸化形式,并且在信息素作用下或显性STE11蛋白共表达时,STE7会发生多重磷酸化。进一步的信号传导似乎需要STE7蛋白激酶活性,因为催化受损的STE7突变体在交配反应中存在缺陷。

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