Schmid Ralf S, Yokota Yukako, Anton E S
Neuroscience Center and Department of Cell and Molecular Physiology, University of North Carolina School of Medicine, Chapel Hill, 27599-7250, USA.
Glia. 2006 Mar;53(4):345-51. doi: 10.1002/glia.20274.
Radial glia play an essential role in the generation of the cerebral cortex through their function as neuronal precursors and as neuronal migration guides. A molecular marker for radial glia in the developing central nervous system is the brain lipid-binding protein (BLBP). To generate mouse models for the visualization and study of radial glia, we expressed EGFP, EYFP, or dsRed2 in transgenic mice under the control of the BLBP promoter. In these transgenic lines, fluorescent protein expression is restricted to radial glia in the embryonic cortex and to astrocytes in the adult brain. Electroporation of the transgenes into embryonic cortex also resulted in radial glia-specific transgene expression. These BLBP promoter driven transgenic mice and organotypic brain slices expressing different fluorescent markers in a radial glia-specific manner will be useful tools to further study the differentiation and function of radial glia in distinct regions of the developing CNS.
放射状胶质细胞通过作为神经元前体和神经元迁移引导者的功能，在大脑皮质的生成中发挥着至关重要的作用。发育中的中枢神经系统中放射状胶质细胞的一个分子标记是脑脂质结合蛋白（BLBP）。为了生成用于可视化和研究放射状胶质细胞的小鼠模型，我们在BLBP启动子的控制下，在转基因小鼠中表达了增强绿色荧光蛋白（EGFP）、增强黄色荧光蛋白（EYFP）或红色荧光蛋白2（dsRed2）。在这些转基因品系中，荧光蛋白的表达仅限于胚胎皮质中的放射状胶质细胞和成年大脑中的星形胶质细胞。将转基因电穿孔导入胚胎皮质也导致了放射状胶质细胞特异性的转基因表达。这些由BLBP启动子驱动的转基因小鼠和以放射状胶质细胞特异性方式表达不同荧光标记的脑片培养物，将成为进一步研究发育中的中枢神经系统不同区域放射状胶质细胞的分化和功能的有用工具。
