Fujita Nobuyuki, Miyamoto Takeshi, Imai Jun-ichi, Hosogane Naobumi, Suzuki Toru, Yagi Mitsuru, Morita Kozo, Ninomiya Ken, Miyamoto Kana, Takaishi Hironari, Matsumoto Morio, Morioka Hideo, Yabe Hiroo, Chiba Kazuhiro, Watanabe Shinya, Toyama Yoshiaki, Suda Toshio
Department of Cell Differentiation, The Sakaguchi Laboratory, Japan; Department of Orthopedic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Biochem Biophys Res Commun. 2005 Dec 30;338(4):1890-6. doi: 10.1016/j.bbrc.2005.10.166. Epub 2005 Nov 10.
Intervertebral disc (IVD) consists of a soft gelatinous material in its center, the nucleus pulposus (NP), bounded peripherally by fibrocartilage, annulus fibrosus (AF). Despite the number of patients with IVD degeneration, gene expression analysis has not been undertaken in NP and therefore little is known about the molecular markers expressed in NP. Here, we undertook a microarray screen in NP with the other nine tissues to identify the specific cell surface markers for NP. Five membrane associating molecules out of 10,490 genes were identified as highly expressing genes in NP compared with the other tissues. Among them, we identified CD24, a glycosylphosphatidylinositol (GPI) anchor protein as a cell surface marker for NP. CD24 expression was also detected in the herniated NP and chordoma, a malignant primary tumor derived from notochordal cells, while it was absent in chondrosarcoma. Therefore, CD24 is a molecular marker for NP as well as the diseases of IVD.
椎间盘（IVD）中央由柔软的胶状物质髓核（NP）组成，周围由纤维软骨纤维环（AF）包绕。尽管患有椎间盘退变的患者数量众多，但尚未对髓核进行基因表达分析，因此对髓核中表达的分子标志物知之甚少。在此，我们对髓核和其他九个组织进行了微阵列筛选，以鉴定髓核的特异性细胞表面标志物。与其他组织相比，在10490个基因中，有五个膜相关分子被鉴定为髓核中的高表达基因。其中，我们鉴定出糖基磷脂酰肌醇（GPI）锚定蛋白CD24作为髓核的细胞表面标志物。在突出的髓核和脊索瘤（一种源自脊索细胞的恶性原发性肿瘤）中也检测到了CD24的表达，而在软骨肉瘤中则未检测到。因此，CD24是髓核以及椎间盘疾病的分子标志物。
