Berto Daniela, Giani Michele, Taddei Paola, Bottura Giorgio
Istituto Centrale per la Ricerca Scientifica e tecnologica Applicata al Mare, Brondolo, Chioggia, 30015 Italy.
Sci Total Environ. 2005 Dec 15;353(1-3):247-57. doi: 10.1016/j.scitotenv.2005.09.017. Epub 2005 Nov 11.
Vibrational Fourier transform infrared (FTIR) and UV-Visible spectroscopies coupled to thermogravimetry were used to characterise the gelatinous aggregates as well as the humin and humic fractions extracted from the aggregates. Spectroscopic analysis made it possible to identify the main classes of compounds present in the samples. The FTIR spectra of the aggregates showed the presence of carbohydrates, saturated aliphatic compounds, aminic, esteric, amidic (proteins) and ketonic compounds, organic and inorganic phosphates, silica and, in some cases, carbonates. The FTIR spectra of humin and humic acids showed functional groups similar to those found in the spectra of the aggregates. The high aliphatic character and the absence of aromatic compounds observed in many aggregates sampled far from estuarine areas suggest the marine origin of mucilages. These results were also confirmed by the 270/407 nm (A2/A4) and 465/665 nm (E4/E6) absorbance ratio in humic acids.
振动傅里叶变换红外（FTIR）光谱和紫外可见光谱与热重分析法联用，用于表征凝胶状聚集体以及从聚集体中提取的胡敏素和腐殖质组分。光谱分析使得识别样品中存在的主要化合物类别成为可能。聚集体的FTIR光谱显示存在碳水化合物、饱和脂肪族化合物、氨基、酯基、酰胺基（蛋白质）和酮类化合物、有机和无机磷酸盐、二氧化硅，在某些情况下还有碳酸盐。胡敏素和腐殖酸的FTIR光谱显示出与聚集体光谱中发现的类似官能团。在远离河口区域采集的许多聚集体中观察到的高脂肪族特征和芳香族化合物的缺失表明黏液的海洋来源。腐殖酸中270/407 nm（A2/A4）和465/665 nm（E4/E6）吸光度比值也证实了这些结果。
