通过外部检测评估碘十六碳烯酸作为脂肪酸代谢示踪剂的研究：对离体大鼠心脏的研究

Assessment of iodohexadecenoic acid as a tracer of fatty acid metabolism by external detection: a study on isolated rat heart.

作者信息

Keriel C M, Dubois F M, Marti Batlle D S, Pernin C R, Leverve X M, Mathieu J P, Comet M, Cuchet P J

机构信息

Laboratoire de Physiologie Cellulaire Cardiaque, Université Joseph Fourier, Grenoble, France.

出版信息

Int J Rad Appl Instrum B. 1992 Apr;19(3):349-55. doi: 10.1016/0883-2897(92)90120-n.

DOI:10.1016/0883-2897(92)90120-n

PMID:1629024

Abstract

Labelled fatty acids have been proposed to explore cardiac metabolism. For the analysis of the external detection curve obtained with 16-iodo 9-hexadecenoic acid (IHA), we developed a mathematical 4-compartment model with compartments 0, 1, 2 and 3 representing vascular IHA, intracellular IHA, esterified forms and iodide, respectively. This model, used here for isolated rat hearts perfused in a recirculating system, is validated by an intracellular analysis, then tested in various metabolic conditions. Thus, the mathematical analysis of the external detection curve gives us numerical data on IHA metabolism, especially the distribution between degradation and storage. Our results confirm the suitability of IHA for assessing myocardial metabolism.

摘要

标记脂肪酸已被用于探索心脏代谢。为了分析用16-碘-9-十六碳烯酸（IHA）获得的外部检测曲线，我们开发了一个数学四室模型，其中0、1、2和3室分别代表血管IHA、细胞内IHA、酯化形式和碘化物。该模型在此用于在循环系统中灌注的离体大鼠心脏，通过细胞内分析进行验证，然后在各种代谢条件下进行测试。因此，对外部检测曲线的数学分析为我们提供了关于IHA代谢的数值数据，特别是降解和储存之间的分布。我们的结果证实了IHA在评估心肌代谢方面的适用性。