Chung Kenny K K, Dawson Valina L, Dawson Ted M
Institute for Cell Engineering, Department of Neurology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Methods Enzymol. 2005;396:139-50. doi: 10.1016/S0076-6879(05)96014-X.
Parkinson's disease (PD) is a common neurodegenerative disorder characterized by impairment in motor function. PD is mostly sporadic, but rare familial cases are also found. The exact pathogenic mechanism is not fully understood, but both genetic and environmental factors are known to be important contributors. In particular, oxidative stress mediated through nitric oxide (NO) is believed to be a prime suspect in the development of PD. NO can exert its effect by modifying different biological molecules, and one of these modifications is through S-nitrosylation. Because of the liable nature of S-nitrosylation, a number of methods are often used to study this modification. We have successfully employed some of these methods and showed that a familial related protein, parkin, can be S-nitrosylated and provide a common pathogenic mechanism for sporadic and familial PD.
帕金森病（PD）是一种常见的神经退行性疾病，其特征在于运动功能受损。PD大多为散发性，但也发现有罕见的家族性病例。确切的致病机制尚未完全了解，但已知遗传和环境因素都是重要的促成因素。特别是，通过一氧化氮（NO）介导的氧化应激被认为是PD发展的主要嫌疑因素。NO可以通过修饰不同的生物分子来发挥其作用，其中一种修饰是通过S-亚硝基化。由于S-亚硝基化的易变性质，通常使用多种方法来研究这种修饰。我们已经成功应用了其中一些方法，并表明一种家族相关蛋白——帕金蛋白，可以被S-亚硝基化，并为散发性和家族性PD提供一种共同的致病机制。
