依克多因对大肠杆菌的渗透保护作用：摄取与积累特性
Osmoprotection of Escherichia coli by ectoine: uptake and accumulation characteristics.
作者信息
Jebbar M, Talibart R, Gloux K, Bernard T, Blanco C
机构信息
Centre National de la Recherche Scientifique URA 256, Laboratoire de Génétique et Physiologie Microbiennes, Rennes, France.
出版信息
J Bacteriol. 1992 Aug;174(15):5027-35. doi: 10.1128/jb.174.15.5027-5035.1992.
Abstract
Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidinecarboxylic acid) is a cyclic amino acid, identified as a compatible solute in moderately halophilic bacteria. Exogenously provided ectoine was found to stimulate growth of Escherichia coli in media of inhibitory osmotic strength. The stimulation was independent of any specific solute, electrolyte or nonelectrolyte. It is accumulated in E. coli cells proportionally to the osmotic strength of the medium, and it is not metabolized. Its osmoprotective ability was as potent as that of glycine betaine. The ProP and ProU systems are both involved in ectoine uptake and accumulation in E. coli. ProP being the main system for ectoine transport. The intracellular ectoine pool is regulated by both influx and efflux systems.
摘要
依克多因（1,4,5,6-四氢-2-甲基-4-嘧啶羧酸）是一种环状氨基酸，被鉴定为中度嗜盐细菌中的相容性溶质。研究发现，外源提供的依克多因可刺激大肠杆菌在具有抑制性渗透强度的培养基中生长。这种刺激与任何特定的溶质、电解质或非电解质无关。它在大肠杆菌细胞中的积累与培养基的渗透强度成正比，且不会被代谢。其渗透保护能力与甘氨酸甜菜碱相当。ProP和ProU系统都参与了依克多因在大肠杆菌中的摄取和积累。ProP是依克多因运输的主要系统。细胞内的依克多因库由流入和流出系统共同调节。
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