Kives J, Guadarrama D, Orgaz B, Rivera-Sen A, Vazquez J, SanJose C
Departamento de Nutrición, Bromatología y Tecnología de Alimentos, Universidad Complutense de Madrid, 28040, Madrid, Spain.
J Dairy Sci. 2005 Dec;88(12):4165-71. doi: 10.3168/jds.S0022-0302(05)73102-7.
Three Lactococcus lactis ssp. cremoris isolates from refrigerated bulk raw milk were cultured separately and in association with a known psychrotrophic dairy Pseudomonas fluorescens strain, in skim UHT milk for 72 h at 7 degrees C, to determine mutual influences in both the planktonic and biofilm phases. Two levels of inoculum of each culture partner were combined. Protocooperation and commensalism cases were found, all of them in the biofilm phase. Type and intensity of the interactions depended on Lactococcus strain and on the cell density of each partner. Maximum enhancement of attachment was observed to be approximately 100-fold for P. fluorescens and 20,000-fold for one of the L. lactis strains. Confocal scanning laser microscopy images show compact masses of Pseudomonas trapping lactococci cells in cooperative biofilms.
从冷藏的散装原料奶中分离出的三株乳酸乳球菌乳脂亚种菌株，分别进行培养，并与一株已知的嗜冷性乳制品荧光假单胞菌菌株共同培养，在7℃下于超高温灭菌脱脂奶中培养72小时，以确定浮游和生物膜阶段的相互影响。每个培养伙伴采用两种接种水平进行组合。发现了原合作和共生情况，所有这些情况都发生在生物膜阶段。相互作用的类型和强度取决于乳酸乳球菌菌株以及每个伙伴的细胞密度。荧光假单胞菌的附着增强最大值约为100倍，而其中一株乳酸乳球菌菌株的附着增强最大值约为20000倍。共聚焦扫描激光显微镜图像显示，在合作生物膜中，假单胞菌紧密聚集，捕获了乳酸乳球菌细胞。
