Liesack W, Stackebrandt E
Department of Microbiology, University of Queensland, St. Lucia, Australia.
J Bacteriol. 1992 Aug;174(15):5072-8. doi: 10.1128/jb.174.15.5072-5078.1992.
A molecular ecological study was performed on an Australian soil sample to unravel a substantial portion of the bacterial diversity. A large fragment of the 16S rRNA gene was amplified, using DNA isolated by lysing the microorganisms directly within the soil matrix, and a clone library was generated. Comparative sequence analysis of 30 clones and dot blot hybridization of 83 additional clones with defined oligonucleotide probes revealed the presence of three major groups of prokaryotes of the domain Bacteria. The first one comprises 57 clones that indicate relatives of nitrogen-fixing bacteria of the alpha-2 subclass of the class Proteobacteria; the second group of 7 clones originates from members of the order Planctomycetales that, however, reveal no close relationship to any of the described Planctomycetales species; 22 clones of the third group are indicative of members of a novel main line of descent, sharing a common ancestry with members of planctomycetes and chlamydiae.
对一份澳大利亚土壤样本进行了分子生态学研究，以揭示很大一部分细菌多样性。利用直接在土壤基质中裂解微生物分离得到的DNA，扩增了16S rRNA基因的大片段，并构建了一个克隆文库。对30个克隆的比较序列分析以及用特定寡核苷酸探针与另外83个克隆进行的斑点杂交，揭示了细菌域中三大类原核生物的存在。第一类包括57个克隆，表明它们是变形菌纲α-2亚类中固氮细菌的亲缘种；第二类有7个克隆，源自浮霉菌目成员，但与任何已描述的浮霉菌目物种均无密切关系；第三类的22个克隆表明是一个新的主要谱系的成员，与浮霉菌和衣原体成员有着共同的祖先。
